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561511 SENP8, His•Tag®, Human, Recombinant, E. coli

SENP8, His•Tag®, Human, Recombinant, E. coli MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
561511
  
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Přehled

Replacement Information
Description
Overview

This product has been discontinued.





Full length recombinant, human SENP8 fused at the N-terminus to a His•Tag® sequence and expressed in E. coli. Structural and biological studies have shown that SENP8 is a highly specific de-NEDDylating enzyme. The protease is useful for the study of SENP8 and peptidase activity and for inhibitor screening.
Catalogue Number561511
Brand Family Calbiochem®
References
ReferencesDrag, M., et al. 2008. Biochem J. 409, 461.
Xirodimas, D.P., et al. 2008. EMBO Rep. 9, 280.
Mikolajczyk, J., et al. 2007. J Biol. Chem. 282, 26217.
Mendoza, H.M., et al. 2003.J Biol Chem. 2003. 278, 25637.
Product Information
ActivityEC₅₀≥0.9 nM as measured in an endopeptidase assay using the substrate Nedd8 per Mikolajczyk, et al.
FormLiquid
FormulationIn 200 mM Imidazole, 100 mM NaCl, 50 mM HEPES, 5% Sucrose, pH 7.8.
Applications
Biological Information
Purity≥90% by SDS-PAGE
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
561511 0

Documentation

SENP8, His•Tag®, Human, Recombinant, E. coli Certificates of Analysis

TitleLot Number
561511

References

Přehled odkazů
Drag, M., et al. 2008. Biochem J. 409, 461.
Xirodimas, D.P., et al. 2008. EMBO Rep. 9, 280.
Mikolajczyk, J., et al. 2007. J Biol. Chem. 282, 26217.
Mendoza, H.M., et al. 2003.J Biol Chem. 2003. 278, 25637.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision06-March-2009 JSW
DescriptionFull length recombinant, human SENP8 fused at the N-terminus to a His•Tag® sequence and expressed in E. coli. Structural and biological studies have shown that SENP8 is a highly specific de-NEDDylating enzyme. The protease is useful for the study of SENP8 and peptidase activity and for inhibitor screening.
FormLiquid
FormulationIn 200 mM Imidazole, 100 mM NaCl, 50 mM HEPES, 5% Sucrose, pH 7.8.
Concentration Label Please refer to vial label for lot-specific concentration
Purity≥90% by SDS-PAGE
ActivityEC₅₀≥0.9 nM as measured in an endopeptidase assay using the substrate Nedd8 per Mikolajczyk, et al.
Storage Avoid freeze/thaw
≤ -70°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Toxicity Standard Handling
ReferencesDrag, M., et al. 2008. Biochem J. 409, 461.
Xirodimas, D.P., et al. 2008. EMBO Rep. 9, 280.
Mikolajczyk, J., et al. 2007. J Biol. Chem. 282, 26217.
Mendoza, H.M., et al. 2003.J Biol Chem. 2003. 278, 25637.