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555460 Rho Inhibitor, Rhosin - Calbiochem

Rho Inhibitor, Rhosin, CAS 1173671-63-0, is a cell-permeable compound that directly targets Rho GEF binding domain (Kd = 354 nM for RhoA), thereby prevents Rho from interacting with its GEFs.

Rho Inhibitor, Rhosin - Calbiochem MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: G04, (2R)-2-Amino-3-(1H-indol-3-yl)-Nʹ-((1E)-quinoxalin-6-ylmethylidene)propanehydrazide

555460
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Products

Katalogové čísloBalení ks/bal.
555460-25MG Skleněná láhev 25 mg
Description
OverviewA cell-permeable (quinoxalinylmethylidene-D-Tryptophanyl)hydrazide that directly targets Rho GEF binding domain (Kd = 354 nM for RhoA), thereby prevents Rho from interacting with its GEFs (including LARG, DBL, LBC, p115 RhoGEF, and PDZ RhoGEF), but not p190RhoGAP, RhoGDI, or its effectors (ROCK, mDia, PKN, Rhoteckin). Shown to inhibit serum-induced RhoA, RhoB, and RhoC, but not Cdc42 or Rac1, activation, MLC & PAK phosphorylation, as well as stress fiber & focal complex formation in serum-starved NIH3T3 cultures (Effective conc. 30 µM) in a reversible manner.
Catalogue Number555460
Brand Family Calbiochem®
SynonymsG04, (2R)-2-Amino-3-(1H-indol-3-yl)-Nʹ-((1E)-quinoxalin-6-ylmethylidene)propanehydrazide
References
ReferencesShang, X., et al. 2012. Chem. Biol. 19, 699.
Product Information
CAS number1173671-63-0
FormOrange brown powder
Hill FormulaC₂₀H₁₈N₆O
Chemical formulaC₂₀H₁₈N₆O
ReversibleY
Structure formula ImageStructure formula Image
Quality LevelMQ100
Applications
ApplicationRho Inhibitor, Rhosin, CAS 1173671-63-0, is a cell-permeable compound that directly targets Rho GEF binding domain (Kd = 354 nM for RhoA), thereby prevents Rho from interacting with its GEFs.
Biological Information
Primary TargetRho GTPase
Primary Target K<sub>i</sub>354 nM for RhoA
Purity≥93% by HPLC
Physicochemical Information
Cell permeableY
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +2°C to +8°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
555460-25MG 04055977268379

Documentation

Rho Inhibitor, Rhosin - Calbiochem MSDS

Title

Safety Data Sheet (SDS) 

Rho Inhibitor, Rhosin - Calbiochem Certificates of Analysis

TitleLot Number
555460

References

Přehled odkazů
Shang, X., et al. 2012. Chem. Biol. 19, 699.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision14-July-2014 JSW
SynonymsG04, (2R)-2-Amino-3-(1H-indol-3-yl)-Nʹ-((1E)-quinoxalin-6-ylmethylidene)propanehydrazide
DescriptionA cell-permeable (quinoxalinylmethylidene-D-tryptophanyl)hydrazide that directly targets Rho GEF binding domain (Kd = 354 nM for RhoA), thereby prevents Rho from interacting with its GEFs (including LARG, DBL, LBC, p115 RhoGEF, and PDZ RhoGEF), but not p190RhoGAP, RhoGDI, or its effectors (ROCK, mDia, PKN, Rhoteckin). Shown to suppress mammospheres formation in MCF7, but not the non-tumorigenic MCF10A, cultures and inhibit serum-induced RhoA, RhoB, and RhoC, but not Cdc42 or Rac1, activation, MLC & PAK phosphorylation, as well as stress fiber and focal complex formation in serum-starved NIH3T3 cultures (Effective conc. 30 µM) in a reversible manner. RhoA inactivation by G04 treatment (30 µM) is also demonstrated to promote neurite outgrowth and branching from NGF-treated PC12 cells.
FormOrange brown powder
Intert gas (Yes/No) Packaged under inert gas
CAS number1173671-63-0
Chemical formulaC₂₀H₁₈N₆O
Structure formulaStructure formula
Purity≥93% by HPLC
SolubilityDMSO (10 mg/ml)
Storage Protect from light
+2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Toxicity Standard Handling
ReferencesShang, X., et al. 2012. Chem. Biol. 19, 699.