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HTS046RTA Ready-to-Assay™ ETB Endothelin Receptor Frozen Cells

Human ETB GPCR frozen cells for Calcium Flux FLIPR Assays.

Host Cell: CHO (Chinese hamster ovary cells) Entrez Gene Number: NM_000115
HTS046RTA
1 kit  
Purchase on Sigma-Aldrich

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Speciální nabídky

This product requires a User Agreement (Label License). Please see product datasheet for further information

Tabulka spec. kláve

SpeciesHost CellsProtein Target
Human CHO (Chinese hamster ovary cells)ETB
Description
Catalogue NumberHTS046RTA
Trade Name
  • Ready-to-Assay
DescriptionReady-to-Assay™ ETB Endothelin Receptor Frozen Cells
Background InformationMillipore’s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.

The endothelin family of peptides are potent vasoconstrictors synthesized by endothelial cells in both constitutive and inducible pathways. The biological actions of endothelins are mediated by two GPCRs, ETA and ETB (Davenport, 2002). Whereas ETA is the predominant receptor on smooth muscle cells and thus is the primary mediator of the vasoconstrictor activity, ETB is found on endothelial cells and mediates vasodilation (Nilsson et al., 1997). In addition, ETB has also been linked to renal failure, congestive heart failure, atherosclerosis, and pulmonary hypertension (D’Orléans-Juste, et al., 2002). A mutation in ETB has been found to cause Hirschsprung disease-2, a degenerative disease of the digestive tract (Puffenberger et al, 1994). Millipore’s cloned human ETB-expressing cell line is made in the CHO host, which supports high levels of recombinant ETB expression on the cell surface for functional detection via the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists, and modulators at ETB.
References
Product Information
Components
  • Pack contains 1 vial of mycoplasma-free cells, 1 ml per vial.
  • Fifty (50) mL of Media Component.
Detection methodFluorescent
Quality LevelMQ300
Applications
ApplicationHuman ETB GPCR frozen cells for Calcium Flux FLIPR Assays.
Key Applications
  • Calcium Flux Assays
Biological Information
Entrez Gene Number
GPCR ClassA
Host CellsCHO (Chinese hamster ovary cells)
Protein TargetETB
Target Sub-FamilyEndothelin
Cell Line Type
  • GPCR Cell Lines
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Packaging Information
Material Size1 kit
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
HTS046RTA 04053252377914

Documentation

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Ready-to-Assay™

Kategorie

Life Science Research > Cell Culture and Systems > Cell Lines > GPCR Frozen Cells
Life Science Research > Drug Discovery and Development > GPCR Cell Lines & Assays > GPCR Frozen Cells