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SCM009 Rat Neural Stem Cell Expansion Medium

The Rat Neural Stem Cell Expansion Medium is provided as a two-component system that is convenient & easy to use.

Rat Neural Stem Cell Expansion Medium MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Stem Cell Culture
SCM009
1 kit  
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Key Applications
Cult
Description
Catalogue NumberSCM009
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionRat Neural Stem Cell Expansion Medium
OverviewThe Rat Neural Stem Cell Expansion Medium is provided as a two-component system that is convenient and easy to use. This product includes the Neural Stem Cell Basal Medium, a defined serum-free medium that has been optimized for the growth and in vitro differentiation of neural stem cells derived from rodents. A solution of basic FGF-2 growth factor is also provided separately, which when added to the Neural Stem Cell Basal Medium allows for the growth and proliferation of rat neural stem cells. Withdrawal of bFGF-2 from the Neural Stem Cell Basal Medium will result in the spontaneous differentiation of rat neural stem cells.
Materials Required but Not Delivered• Cryopreserved Rat Cortical Neural Stem Cells (Millipore Catalog No. SCR022)
• Neural Stem Cell Characterization Kit (Millipore Catalog No. SCR019)
• Poly-L-ornithine (Sigma Catalog No. P3655)
• Laminin (Millipore Catalog No. CC095)
• Accutase solution (Millipore Catalog No. SCR005)
Product Information
Components
  • Neural Stem Cell Basal Medium (Cat.# SCM003), 500 mL
  • bFGF, 10 ug
Quality LevelMQ100
Applications
ApplicationThe Rat Neural Stem Cell Expansion Medium is provided as a two-component system that is convenient & easy to use.
Key Applications
  • Stem Cell Culture
Biological Information
Media FormLiquid
Stem Cell Type
  • Neural Stem Cells
Product Usage Statements
Quality AssuranceEach lot of medium is tested for the optimal cell growth, proliferation and differentiation of mouse neural stem cells. Sterility Testing: Negative; Osmolarity: 299; pH: 7.4
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsPart number SCM003, the Neural Stem Cell Basal Medium should be stored at -20°C until ready to use. Upon thawing, the basal medium should be stored at 2-8°C for up to one month.


FGF-2 (10 µg, lyophilized) should be reconstituted with 100 µL 5 mM Tris-HCL, pH 7.6 for a final concentration of 100 µg/mL. Dispense into aliquots to avoid repeated freeze-thaw. Store at -20°C for up to six months. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or -20°C for future use. Multiple freeze/thaw cycles will result in significant loss of activity.
Packaging Information
Material Size1 kit
Global Trade Item Number
Katalogové číslo GTIN
SCM009 04053252663123