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556785 RNase T₁, Aspergillus oryzae

RNase T₁, Aspergillus oryzae MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Ribonuclease T₁

556785
  
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Description
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Native RNase T1 from Aspergillus oryzae. Guanylate-specific ribonuclease that hydrolyzes the 3′-phosphate of guanylate residues in polynucleotides. Guanosine-3′-phosphates and oligonucleotides with guanosine-3′-phosphate terminal groups are the final products. Note: 1 TU = 1000 units.
Catalogue Number556785
Brand Family Calbiochem®
SynonymsRibonuclease T₁
References
ReferencesWaltz, F.G., Jr. 1997. Biochim. Biophys. Acta 1350, 183.
Plaza del Pino, I.M., et al. 1992. Biochemistry 31, 11196.
Uchimavu, T., et al. 1992. Biochem. Biophys. Res. Commun. 187, 1523.
Zegers, I., et al. 1992. Biochemistry 31, 11317.
Egami, F., et al. 1964. Prog. Nucleic Acid Res. Mol. Biol. 3, 59.
Product Information
CAS number9026-12-4
Activity≥200 units/mg dry weight
Unit of DefinitionAccording to Egami et al. (1964) one unit will increase the absorbance of the acid-soluble fraction of the 0.3% yeast RNA solution by A₂₆₀ = 1 in 15 min at 37°C, pH 7.5 in a reaction mixture of 1 ml. One Calbiochem unit equals 30 Egami units.
EC number3.1.27.3
FormLyophilized
Applications
Biological Information
Physicochemical Information
ContaminantsDNase, mono- and diesterase: ≤0.02%
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
RTECSRF0753000
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
556785 0

Documentation

RNase T₁, Aspergillus oryzae Certificates of Analysis

TitleLot Number
556785

References

Přehled odkazů
Waltz, F.G., Jr. 1997. Biochim. Biophys. Acta 1350, 183.
Plaza del Pino, I.M., et al. 1992. Biochemistry 31, 11196.
Uchimavu, T., et al. 1992. Biochem. Biophys. Res. Commun. 187, 1523.
Zegers, I., et al. 1992. Biochemistry 31, 11317.
Egami, F., et al. 1964. Prog. Nucleic Acid Res. Mol. Biol. 3, 59.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision06-June-2008 JSW
SynonymsRibonuclease T₁
DescriptionNative RNase T1 from Aspergillus oryzae. Ribonuclease that cleaves the phosphodiester bond between the 3'-phosphate of guanylate residues and the 5'-hydroxy group of the adjacent nucleotide. The reaction, which involves the formation of 2'3'-cyclic guanylate intermediate, splits RNA into 3'-GMP and oligonucleo-tides with a terminal 3'-GMP.

Note: 1 TU = 1000 units.
FormLyophilized
CAS number9026-12-4
RTECSRF0753000
EC number3.1.27.3
ContaminantsDNase, mono- and diesterase: ≤0.02%
Activity≥200 units/mg dry weight
Unit definitionAccording to Egami et al. (1964) one unit will increase the absorbance of the acid-soluble fraction of the 0.3% yeast RNA solution by A₂₆₀ = 1 in 15 min at 37°C, pH 7.5 in a reaction mixture of 1 ml. One Calbiochem unit equals 30 Egami units.
SolubilityH₂O and Aqueous Buffers
Storage +2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Toxicity Standard Handling
Merck USA index14, 8202
ReferencesWaltz, F.G., Jr. 1997. Biochim. Biophys. Acta 1350, 183.
Plaza del Pino, I.M., et al. 1992. Biochemistry 31, 11196.
Uchimavu, T., et al. 1992. Biochem. Biophys. Res. Commun. 187, 1523.
Zegers, I., et al. 1992. Biochemistry 31, 11317.
Egami, F., et al. 1964. Prog. Nucleic Acid Res. Mol. Biol. 3, 59.