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656000 PhosphoDetect™ Anti-Tyk2 (pTyr1054/1055) Rabbit pAb

This PhosphoDetect™ Anti-Tyk2 (pTyr1054/1055) Rabbit pAb is validated for use in Immunoblotting for the detection of Tyk2 (pTyr1054/1055).

PhosphoDetect™ Anti-Tyk2 (pTyr1054/1055) Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
656000
  
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Přehled

Replacement Information

Tabulka spec. kláve

Host
Rb
Description
Overview

This product has been discontinued.

Recognizes Tyk2 phosphorylated at Tyr1054/1055.
Catalogue Number656000
Brand Family Calbiochem®
Application Data
Detection of human Tyk2, phosphorylated on Tyr1054/1055, by immunoblotting. Samples: HeLa cell extracts untreated or treated with IFN-alpha. Primary antibody: PhosphoDetect™ Anti-Tyk2 (pTyr1054/1055) Rabbit pAb (Cat. No. 656000) (1:1000). Detection: chemiluminescence.
References
ReferencesGauzzi, M.C., et al. 1996. J. Biol. Chem. 271, 20494.
Colamonici, O.R., et al. 1994. J. Biol. Chem. 269, 3518.
Product Information
FormLiquid
FormulationIn 150 mM NaCl, 10 mM HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5.
PreservativeNone
Applications
Key Applications Immunoblotting (Western Blotting)
Application NotesImmunoblotting (1:1000, chemiluminescence)
Application CommentsTo reduce basal levels of Tyk2 phosphorylation, incubate cells in low-serum (0.5%) medium for 2 days. Change to fresh serum-free medium immediately before treatment. Does not recognize nonphosphorylated Tyk2. Variables associated with assay conditions will dictate the optimal working dilution.
Biological Information
Immunogena synthetic peptide corresponding to amino acids surrounding the Tyr1054/1055 phosphorylation sites of human Tyk2, conjugated to KLH
ImmunogenHuman
HostRabbit
IsotypeIgG
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
656000 0

Documentation

PhosphoDetect™ Anti-Tyk2 (pTyr1054/1055) Rabbit pAb MSDS

Title

Safety Data Sheet (SDS) 

PhosphoDetect™ Anti-Tyk2 (pTyr1054/1055) Rabbit pAb Certificates of Analysis

TitleLot Number
656000

References

Přehled odkazů
Gauzzi, M.C., et al. 1996. J. Biol. Chem. 271, 20494.
Colamonici, O.R., et al. 1994. J. Biol. Chem. 269, 3518.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision16-August-2007 RFH
ApplicationImmunoblotting (1:1000, chemiluminescence)
Application Data
Detection of human Tyk2, phosphorylated on Tyr1054/1055, by immunoblotting. Samples: HeLa cell extracts untreated or treated with IFN-alpha. Primary antibody: PhosphoDetect™ Anti-Tyk2 (pTyr1054/1055) Rabbit pAb (Cat. No. 656000) (1:1000). Detection: chemiluminescence.
DescriptionProtein A and immunoaffinity purified rabbit polyclonal antibody. Recognizes Tyk2 phosphorylated at Tyr1054/1055.
BackgroundTyk2, a member of the Janus Kinase (JAK) family of kinases, is associated with the IFN-α/β receptor. Upon ligand binding, Tyk2 is activated by phosphorylation on Tyr1054 and/or Tyr1055, initiating the IFN-α/β signaling cascade.
HostRabbit
Immunogen speciesHuman
Immunogena synthetic peptide corresponding to amino acids surrounding the Tyr1054/1055 phosphorylation sites of human Tyk2, conjugated to KLH
IsotypeIgG
Specieshuman
FormLiquid
FormulationIn 150 mM NaCl, 10 mM HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5.
Concentration Label Please refer to vial label for lot-specific concentration
PreservativeNone
CommentsTo reduce basal levels of Tyk2 phosphorylation, incubate cells in low-serum (0.5%) medium for 2 days. Change to fresh serum-free medium immediately before treatment. Does not recognize nonphosphorylated Tyk2. Variables associated with assay conditions will dictate the optimal working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesGauzzi, M.C., et al. 1996. J. Biol. Chem. 271, 20494.
Colamonici, O.R., et al. 1994. J. Biol. Chem. 269, 3518.