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482737 PhosphoDetect™ Anti-Nitric Oxide Synthase, Endothelial (pSer¹¹⁷⁷) Mouse mAb (15E2)

PhosphoDetect™ Anti-Nitric Oxide Synthase, Endothelial (pSer¹¹⁷⁷) Mouse mAb (15E2) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Activated eNOS (pSer¹¹⁷⁷)

482737
  
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Description
Overview

This product has been discontinued.





Recognizes the ~140 kDa eNOS protein phosphorylated at Ser1177 in endothelial cells.
Catalogue Number482737
Brand Family Calbiochem®
SynonymsAnti-Activated eNOS (pSer¹¹⁷⁷)
Application Data
Detection of mouse endothelial nitric oxide synthase, phosphorylated on Ser1177, by immunoblotting. Samples: Whole cell lysates from bEnd 3 cells left untreated (lane 1) and pervanadate treated (lane 2). Primary antibody: PhosphoDetect™ Anti-Nitric Oxide Synthase, Endothelial (pSer1177) Mouse mAb (15E2) (Cat. No. 482737) (1 µg/ml). Detection: chemiluminescence.
References
Product Information
FormLiquid
FormulationIn PBS, PEG, sucrose, 50% glycerol.
Preservative≤0.1% sodium azide
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Application NotesELISA (0.05 µg/ml)
Immunoblotting (1-2 µg/ml)
Application CommentsVariables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Ser¹¹⁷⁷ phosphorylation site of human endothelial nitric oxide synthase (eNOS)
ImmunogenHuman
Clone15E2
HostMouse
IsotypeIgG₁
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
482737 0

Documentation

PhosphoDetect™ Anti-Nitric Oxide Synthase, Endothelial (pSer¹¹⁷⁷) Mouse mAb (15E2) Certificates of Analysis

TitleLot Number
482737
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision21-May-2010 JSW
SynonymsAnti-Activated eNOS (pSer¹¹⁷⁷)
ApplicationELISA (0.05 µg/ml)
Immunoblotting (1-2 µg/ml)
Application Data
Detection of mouse endothelial nitric oxide synthase, phosphorylated on Ser1177, by immunoblotting. Samples: Whole cell lysates from bEnd 3 cells left untreated (lane 1) and pervanadate treated (lane 2). Primary antibody: PhosphoDetect™ Anti-Nitric Oxide Synthase, Endothelial (pSer1177) Mouse mAb (15E2) (Cat. No. 482737) (1 µg/ml). Detection: chemiluminescence.
DescriptionMouse monoclonal antibody purified from serum-free cell culture supernatant by thiophilic adsorption and size exclusion chromatography. Recognizes the ~140 kDa eNOS protein phosphorylated at Ser1177.
BackgroundEndothelial NO synthase produces nitric oxide, which is crucial to cardiovascular homeostasis.
HostMouse
Immunogen speciesHuman
Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Ser¹¹⁷⁷ phosphorylation site of human endothelial nitric oxide synthase (eNOS)
Clone15E2
IsotypeIgG₁
Specieshuman, mouse
FormLiquid
FormulationIn PBS, PEG, sucrose, 50% glycerol.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsVariables associated with assay conditions will dictate the proper working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling