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539655 PKCβI, Human, Recombinant, S. frugiperda

539655
  
Purchase on Sigma-Aldrich

Přehled

Replacement Information
Description
Overview

This product has been discontinued.



Full-length, recombinant, human PKCβI expressed in S. frugiperda insect cells. Suitable for studies of PKCβI and its regulation. PKCβI is a Ca2+ and phospholipid-dependent enzyme.

Catalogue Number539655
Brand Family Calbiochem®
SynonymsProtein Kinase CβI
References
ReferencesNewton, A.C. 1995. J. Biol. Chem. 270, 28495.
Dekker, L.V. and Parker, P.J. 1994. Trends Biochem. Sci. 19, 73.
Nishizuka, Y. 1992. Science 258, 607.
Bell, R.M. and Burns, D.J. 1991. J. Biol. Chem. 266, 4661.
Product Information
Unit of DefinitionOne unit is defined as the amount of enzyme that will transfer 1.0 nmol of phosphate to histone H1 per min at 30°C, pH 7.4.
FormLiquid
FormulationIn 100 mM NaCl, 20 mM HEPES, 5 mM DTT, 2 mM EDTA, 2 mM EGTA, 50% glycerol, 0.05% TRITON® X-100 Detergent, pH 7.4.
Quality LevelMQ100
Applications
Biological Information
Purity≥95% by SDS-PAGE
Specific Activity≥800 units/mg protein
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
539655 0

Documentation

PKCβI, Human, Recombinant, S. frugiperda MSDS

Title

Safety Data Sheet (SDS) 

PKCβI, Human, Recombinant, S. frugiperda Certificates of Analysis

TitleLot Number
539655

References

Přehled odkazů
Newton, A.C. 1995. J. Biol. Chem. 270, 28495.
Dekker, L.V. and Parker, P.J. 1994. Trends Biochem. Sci. 19, 73.
Nishizuka, Y. 1992. Science 258, 607.
Bell, R.M. and Burns, D.J. 1991. J. Biol. Chem. 266, 4661.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision05-September-2008 RFH
SynonymsProtein Kinase CβI
DescriptionBiologically active full-length recombinant protein kinase CβI (PKCβI) isoform. Prepared from Sf-9 cells, which have been infected with baculovirus containing cDNA for human protein kinase CβI. Suitable for studies of PKCβI and its regulation. PKCβI is a Ca2+ and phospholipid-dependent enzyme.
FormLiquid
FormulationIn 100 mM NaCl, 20 mM HEPES, 5 mM DTT, 2 mM EDTA, 2 mM EGTA, 50% glycerol, 0.05% TRITON® X-100 Detergent, pH 7.4.
Concentration Label Please refer to vial label for lot-specific concentration
Purity≥95% by SDS-PAGE
Specific activity≥800 units/mg protein
Unit definitionOne unit is defined as the amount of enzyme that will transfer 1.0 nmol of phosphate to histone H1 per min at 30°C, pH 7.4.
Storage Avoid freeze/thaw
≤ -70°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Toxicity Standard Handling
ReferencesNewton, A.C. 1995. J. Biol. Chem. 270, 28495.
Dekker, L.V. and Parker, P.J. 1994. Trends Biochem. Sci. 19, 73.
Nishizuka, Y. 1992. Science 258, 607.
Bell, R.M. and Burns, D.J. 1991. J. Biol. Chem. 266, 4661.