PEGA resins

PEGA resins are hydrophilic polymers that consist of 2-acrylamidoprop-1-yl-(2-aminoprop-1-yl) polyethylene glycol₈₀₀ and dimethylacylamide cross-linked with bis 2-acrylamidoprop-1-yl polyethylene glycol₈₀₀ ¹. These supports swell extensively in a wide range of solvents, including water, DMF, DCM, THF and MeOH, and are freely permeable to macromolecules up to 35kD. These characteristics make them ideally suited for the preparation of peptide libraries, affinity purification, and on-resin enzyme assays. These properties have been exploited in the on-resin enzymatic synthesis of glycopeptides²; for determining the inhibitors of subtilisin Carlsberg³, Cruzipain⁴, cysteine proteases⁵, and matrix metalloproteinases⁶; and in studies on protein disulfide isomerases using fluorescence-quenched libraries⁷.

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2. M. Meldal et al. (1994) J. Chem. Soc., Chem. Commun., 1849.
3. M. Meldal et al. (1994) Proc. Natl. Acad. Sci. USA, 91, 3314.
4. M. Meldal, et al. (1998) J. Peptide Sci., 4, 83.
5. P. M. St. Hilaire, et al. (1999) J. Comb. Chem., 1, 509.
6. J. Buchardt, et al. (2000) J. Comb. Chem., 2, 624.
7. J. C. Spetzler, et al. (1998) J. Peptide Sci., 4, 128.