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71346 Origami™ 2(DE3)pLysS Competent Cells - Novagen

Origami 2 strains have mutations in glutathione reducatase(gor) and thioredoxin reductase (trxB), facilitating proper disulfide bond formation. T7 lysozyme expression suppresses basal T7 expression.

Origami™ 2(DE3)pLysS Competent Cells - Novagen MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
71346
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Katalogové čísloBalení ks/bal.
71346-3 Plastová ampulka 0.4 ml
71346-4 Skleněná láhev 1 ml
Description
OverviewOrigami™ 2 host strains are K-12 derivatives that have mutations in both the thioredoxin reductase (trxB) and glutathione reductase (gor) genes, which greatly enhance disulfide bond formation in the E. coli cytoplasm. The Origami 2 strains are kanamycin sensitive; like the original Origami strains, the gor mutation is still selected for by tetracycline. To reduce the possibility of disulfide bond formation between molecules, strains containing mutations in trxB and gor are recommended only for the expression of proteins that require disulfide bond formation for proper folding.

DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.

pLysS strains express T7 lysozyme, which further suppresses basal expression of T7 RNA polymerase prior to induction, thus stabilizing pET recombinants encoding target proteins that affect cell growth and viability.
Genotype: Δ(ara-leu)7697 ΔlacX74 ΔphoA PvuII phoR araD139 ahpC galE galK rpsL F′[lac+ lacIq pro] (DE3) gor522::Tn10 trxB pLysS (CamR, StrR, TetR)


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Catalogue Number71346
Brand Family Novagen®
References
Product Information
0.4 ml1 mlComponent
•2 × 0.2 ml5 × 0.2 mlOrigami™ 2(DE3)pLysS Competent Cells
•2 × 2 ml4 × 2 mlSOC Medium
•2 ng2 ngTest Plasmid
Guaranteed efficiency2 × 10⁶ cfu/µg
Quality LevelMQ100
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Multiple Toxicity Values, refer to MSDS
Storage ≤ -70°C
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
71346-3 04055977253337
71346-4 04055977253344