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481909 Niclosamide - CAS 50-65-7 - Calbiochem

A salicylanilide anthelmintic drug that acts as a potent and selective Stat3- and Wnt-signaling pathway inhibitor.

Niclosamide - CAS 50-65-7 - Calbiochem MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: 5-Chloro-N-(2-chloro-4-nitrophenyl)-2-hydroxy-benzamide, Autophagy Inducer III, STAT3 Inhibitor XV, Wnt Pathway Inhibitor XIII, mTOR Inhibitor IX

481909
  
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Přehled

Replacement Information

Tabulka spec. kláve

CAS #Empirical Formula
50-65-7C₁₃H₈Cl₂N₂O₄
Description
OverviewA cell-permeable salicylanilide that, in addition to its well-known antihelmintic efficacy, acts as a mammalian mTORC1, but not mTORC2, signaling inhibitor mechanistically distinct from rapamycin (Cat. Nos. 553210, 553211, & 553212). Likely a direct consequence of autophagy activation, Niclosamide is demonstrated to induce Wnt-independent Frizzled1 and Dishevelled-2 downregulation. Unrelated to its autophagy induction activity, Niclosamide is also shown to inhibit Stat3 signaling (IC50 = 0.25 µM in HeLa reporter assays). Efficiently inhibits breast cancer stem-like cells in vitro and in vivo.
Catalogue Number481909
Brand Family Calbiochem®
Synonyms5-Chloro-N-(2-chloro-4-nitrophenyl)-2-hydroxy-benzamide, Autophagy Inducer III, STAT3 Inhibitor XV, Wnt Pathway Inhibitor XIII, mTOR Inhibitor IX
References
ReferencesWang, Y.C., et al. 2013. PLoS ONE 8, e74538.
Chen, W., et al. 2010. Am. J. Physiol. Gastrointest. Liver Physiol. 299, G293.
Gies, E., et al. 2010. PLoS ONE 5, e14410.
Ren, X., et al. 2010. ACS Med. Chem. Lett. 1, 454.
Chen, M., et al. 2009. Biochemistry 48, 10267.
Product Information
CAS number50-65-7
FormPale yellow solid
Hill FormulaC₁₃H₈Cl₂N₂O₄
Chemical formulaC₁₃H₈Cl₂N₂O₄
Structure formula ImageStructure formula Image
Quality LevelMQ100
Applications
Biological Information
Purity≥97% by HPLC
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Shipped at Ambient Temperature or with Blue Ice or with Dry Ice
Toxicity Regulatory Review
Storage +2°C to +8°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C. Stock solutions are stable for up to 6 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
481909 0

Documentation

Niclosamide - CAS 50-65-7 - Calbiochem MSDS

Title

Safety Data Sheet (SDS) 

Niclosamide - CAS 50-65-7 - Calbiochem Certificates of Analysis

TitleLot Number
481909

References

Přehled odkazů
Wang, Y.C., et al. 2013. PLoS ONE 8, e74538.
Chen, W., et al. 2010. Am. J. Physiol. Gastrointest. Liver Physiol. 299, G293.
Gies, E., et al. 2010. PLoS ONE 5, e14410.
Ren, X., et al. 2010. ACS Med. Chem. Lett. 1, 454.
Chen, M., et al. 2009. Biochemistry 48, 10267.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision26-March-2012 JSW
Synonyms5-Chloro-N-(2-chloro-4-nitrophenyl)-2-hydroxy-benzamide, Autophagy Inducer III, STAT3 Inhibitor XV, Wnt Pathway Inhibitor XIII, mTOR Inhibitor IX
DescriptionA cell-permeable salicylanilide that, in addition to its well-known antihelmintic efficacy and use in tapeworm treatment, acts as a mammalian mTORC1, but not mTORC2, signaling inhibitor mechanistically distinct from rapamycin (Cat. Nos. 553210, 553211, & 553212), causing MCF-7 autophagy activation under nutrient-rich conditions (by 15-fold with 4h 10 µM treatment), SH-SY5Y lysosome redistribution (10 µM for 8 h), and dissipation/clearance of ubiquinated protein aggregates upon proteasome inhibition in a reversible, time- and dose-dependent manner. Likely a direct consequence of autophagy activation, Niclosamide is demonstrated to induce Wnt-independent Frizzled1 endocytosis (t1/2 = 2.4 h; effective conc. <2 µM) and Dishevelled-2 downregulation (IC50 ~1 µM), effectively preventing Wnt3a from stimulating HEK293 cellular LEF/TCF transcription activity (IC50 = 0.5 µM TOPFlash reporter assays). Unrelated to its autophagy induction activity, Niclosamide is also shown to inhibit Stat3 signaling (IC50 = 0.25 µM in HeLa-based pLucTKS3 reporter assays) by suppressing the phosphorylation of Jak1 Tyr1022/1023 (2 h treatment at 0.5 to 2.0 µM in Du145 cultures) and Stat3 Tyr705 (by >90% after 24 h treatment at 2 and 5 µM, respectively, in Du145 and HeLa cultures), without affecting the phosphorylation of Stat1 Tyr701, Stat3 Ser727, Stat5 Tyr694, or Jak2 Tyr1007/1008.
FormPale yellow solid
Intert gas (Yes/No) Packaged under inert gas
CAS number50-65-7
Chemical formulaC₁₃H₈Cl₂N₂O₄
Structure formulaStructure formula
Purity≥97% by HPLC
SolubilityDMSO (2.5 mg/ml) or Ethanol (5 mg/ml)
Storage Protect from light
+2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C. Stock solutions are stable for up to 6 months at -20°C.
Toxicity Regulatory Review
ReferencesWang, Y.C., et al. 2013. PLoS ONE 8, e74538.
Chen, W., et al. 2010. Am. J. Physiol. Gastrointest. Liver Physiol. 299, G293.
Gies, E., et al. 2010. PLoS ONE 5, e14410.
Ren, X., et al. 2010. ACS Med. Chem. Lett. 1, 454.
Chen, M., et al. 2009. Biochemistry 48, 10267.