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EZML-82BK Mouse Leptin ELISA- Space Saver (Bulk) Packaging

EZML-82BK
96-well strip plate  
Purchase on Sigma-Aldrich

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Replacement Information

Tabulka spec. kláve

Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
Leptin MELISAFluorescent Colorimetric
Description
Catalogue NumberEZML-82BK
DescriptionMouse Leptin ELISA- Space Saver (Bulk) Packaging
Background InformationLeptin is synthesized in adipocytes as a 16 kDa molecule and the circulating level is directly proportional to the total amount of fat in the body.
References
Product Information
Detection methodFluorescent Colorimetric
Precision, %
  • Inter-assay: 3.0–4.6
  • Intra-assay: 1.1–1.8
Quality LevelMQ200
Applications
ApplicationThis Mouse Leptin ELISA is used to measure & quantify Leptin levels in Metabolism & Endocrine & Neuroscience research.
Key Applications
  • ELISA
Application NotesRoom temperature, 4 hour assay 10 µL sample size
Biological Information
Species Reactivity
  • Mouse
Analytes Available
  • Leptin
Entrez Gene SummaryThis gene encodes a protein that is secreted by white adipocytes, and which plays a major role in the regulation of body weight. This protein, which acts through the leptin receptor, functions as part of a signaling pathway that can inhibit food intake and/or regulate energy expenditure to maintain constancy of the adipose mass. This protein also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing. Mutations in this gene and/or its regulatory regions cause severe obesity, and morbid obesity with hypogonadism. This gene has also been linked to type 2 diabetes mellitus development.
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P41159 # May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass.
SIZE: 167 amino acids; 18641 Da
SUBUNIT: Interacts with SIGLEC6.
SUBCELLULAR LOCATION: Secreted.
DISEASE:SwissProt: P41159 # Defects in LEP may be a cause of autosomal recessive obesity [MIM:601665].
SIMILARITY: SwissProt: P41159 ## Belongs to the leptin family.
Accuracy
  • 87–100%
Physicochemical Information
Sensitivity
  • 0.05 ng/mL
Linearity of Dilution100–111%
Standard Curve Range
  • 0.2–30 ng/mL
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • For research use only. Not for use in diagnostic procedures.
Storage and Shipping Information
Packaging Information
Material Size96-well strip plate
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
EZML-82BK 04053252437038

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Kategorie

Life Science Research > Antibodies and Assays > Immunoassays > Enzyme-linked Immunosorbent Assay (ELISA) > Complete ELISA Kits
Life Science Research > Protein Detection and Quantification > Immunoassays > Enzyme-linked Immunosorbent Assay (ELISA) > Complete ELISA Kits