Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

411493 Mouse Anti-Human IgG₄, Fc Fragment Specific (HP6023) Biotin Conjugate

Mouse Anti-Human IgG₄, Fc Fragment Specific (HP6023) Biotin Conjugate MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
411493
  
Purchase on Sigma-Aldrich

Přehled

Replacement Information
Description
Overview

This product has been discontinued.
Catalogue Number411493
Brand Family Calbiochem®
References
ReferencesHamilton, R.G. 1990. Ann. Biol. Clin. 48, 473.
Fasullo, F.J., et al. 1989. Clin. Chem. 35, 364.
Hamilton, R.G. 1987. Clin. Chem. 33, 1707.
Jefferis, R., et al. 1986. Immunol. Lett. 10, 223.
Product Information
FormLiquid
FormulationIn PBS, 1% BSA, pH 7.4.
PreservativeNone
Applications
Application ReferencesSpecificity by ELISA, chimeric antibodies Hamilton, R.G. 1990. Ann. Biol. Clin. 48, 473. Specificity by ELISA, human myeloma proteins Fasullo, F.J., et al. 1989. Clin. Chem. 35, 364. Jefferis, R., et al. 1986. Immunol. Lett. 10, 223. Enzyme Immunoassay Hamilton, R.G. 1987. Clin. Chem. 33, 1707.
Key Applications Enzyme Immunoassay
Application NotesEnzyme Immunoassay (1:1000, see application references)
Application CommentsAntibody should be titrated for optimal results in individual systems.

Cross-reactivity by ELISA against human myeloma proteins:
Human IgG1: < 0.5%
Human IgG2: < 0.1%
Human IgG3: < 0.5%
Human IgG4: 100%
Human IgM: < 0.1%
Human IgA: < 0.1%
Human IgE: < 0.1%

Cross-reactivity by ELISA against chimeric antibodies:
Human IgG1: < 0.01%
Human IgG2: < 0.01%
Human IgG3: < 0.01%
Human IgG4: 100%
Human IgM: < 0.01%
Human IgA: < 0.01%
Human IgE: < 0.01%
Biological Information
ImmunogenHuman
CloneHP6023
HostMouse
IsotypeIgG₃
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C). Do not freeze/thaw more than 5 times.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
411493 0

Documentation

Mouse Anti-Human IgG₄, Fc Fragment Specific (HP6023) Biotin Conjugate Certificates of Analysis

TitleLot Number
411493

References

Přehled odkazů
Hamilton, R.G. 1990. Ann. Biol. Clin. 48, 473.
Fasullo, F.J., et al. 1989. Clin. Chem. 35, 364.
Hamilton, R.G. 1987. Clin. Chem. 33, 1707.
Jefferis, R., et al. 1986. Immunol. Lett. 10, 223.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision06-August-2007 RFH
ApplicationEnzyme Immunoassay (1:1000, see application references)
DescriptionPurified mouse monoclonal antibody conjugated to biotin. Recognizes human IgG4 Fc fragment.
BackgroundThe HP series of murine human Ig-specific monoclonal antibodies was produced from documented hybridoma cell lines that were developed at the Centers for Disease Control in Atlanta, Georgia. Laboratory analysis of each monoclonal antibody lot is performed in two stages during the production of ascites. First, the cell culture medium is analyzed for the presence and relative amount of human Ig-specific antibody by ELISA before injection of cells into mice. Second, collected ascites are analyzed for potency and antibody specificity using ELISA and isoelectric focusing (IEF), often in combination with an affinity immunoblot. For more information, the Calbiochem® technical monograph, The Human IgG Subclasses, is available free of charge.
HostMouse
Immunogen speciesHuman
CloneHP6023
IsotypeIgG₃
FormLiquid
FormulationIn PBS, 1% BSA, pH 7.4.
Concentration Label Please refer to vial label for lot-specific concentration
PreservativeNone
CommentsAntibody should be titrated for optimal results in individual systems.

Cross-reactivity by ELISA against human myeloma proteins:
Human IgG1: < 0.5%
Human IgG2: < 0.1%
Human IgG3: < 0.5%
Human IgG4: 100%
Human IgM: < 0.1%
Human IgA: < 0.1%
Human IgE: < 0.1%

Cross-reactivity by ELISA against chimeric antibodies:
Human IgG1: < 0.01%
Human IgG2: < 0.01%
Human IgG3: < 0.01%
Human IgG4: 100%
Human IgM: < 0.01%
Human IgA: < 0.01%
Human IgE: < 0.01%
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C). Do not freeze/thaw more than 5 times.
Toxicity Standard Handling
ReferencesHamilton, R.G. 1990. Ann. Biol. Clin. 48, 473.
Fasullo, F.J., et al. 1989. Clin. Chem. 35, 364.
Hamilton, R.G. 1987. Clin. Chem. 33, 1707.
Jefferis, R., et al. 1986. Immunol. Lett. 10, 223.
Application referencesSpecificity by ELISA, chimeric antibodies Hamilton, R.G. 1990. Ann. Biol. Clin. 48, 473. Specificity by ELISA, human myeloma proteins Fasullo, F.J., et al. 1989. Clin. Chem. 35, 364. Jefferis, R., et al. 1986. Immunol. Lett. 10, 223. Enzyme Immunoassay Hamilton, R.G. 1987. Clin. Chem. 33, 1707.