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03-229 LSD1 human recombinant

Human recombinant LSD1 expressed in E. Coli.

LSD1 human recombinant MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Enzyme Assays UniProt Number: O60341
03-229
50 µg  
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Replacement Information

Tabulka spec. kláve

Key ApplicationsSpeciesUni Prot Number
Enzyme AssaysHuman O60341
Description
Catalogue Number03-229
Brand Family Upstate
Trade Name
  • Upstate
DescriptionLSD1 human recombinant
OverviewHuman recombinant LSD1 expressed in E. Coli
Background InformationLSD1 catalyzes the oxidative demethylation of mono- and dimethylated H3K4, producing hydrogen peroxide and formaldehyde in the process. H3K4 methylation is considered a transcription-activating chromatin mark, and in vivo LSD1 is frequently found in association with the transcriptional corepressor protein CoREST and HDACs 1 or 2. LSD1 is inhibited by a number of established monoamine oxidase inhibitor drugs. That and the fact that its expression is elevated in a number of cancers may make it a promising target for drug development.
References
Product Information
FormatPurified
Presentation6 mM Na2HPO4, 1.1 mM K2HPO4, pH 7.2, 82 mM sodium chloride, 1.6 mM KCl and 40% glycerol. Frozen solution.
Quality LevelMQ100
Applications
ApplicationHuman recombinant LSD1 expressed in E. Coli.
Key Applications
  • Enzyme Assays
Application NotesDemethylation enzyme assays
Biological Information
PurityPurity >90% by SDS-PAGE
Specific Activity>20U/μg. One U=1 pmol/min at 30ºC as determined by a Peroxidase-Coupled Assay with a Histone H3 dimethyl lysine 4 peptide.
UniProt Number
Molecular Weight~78 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 12 months at -80ºC from date of receipt. The enzyme is stable on ice for 30 to 60 min, but may lose activity with prolonged storage on ice. It is recommended that thawing and dilution of the enzyme be done within as short a time as possible before start of the assay. The remaining, unused, undiluted enzyme should be refrozen quickly by, for example, snap freezing in a dry/ice ethanol bath or liquid nitrogen. Freezing and storage of diluted enzyme is not recommended.
Packaging Information
Material Size50 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
03-229 04053252550904