Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

PF059 Klenow Enzyme

Klenow Enzyme MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
PF059
  
Purchase on Sigma-Aldrich

Přehled

Replacement Information
Description
Overview

This product has been discontinued.





The large fragment of DNA polymerase I. The Klenow fragment exhibits 5′ to 3′ DNA polymerase and 3′ to 5′ exonuclease activity, but lacks the 5′ to 3′ exonuclease activity of intact DNA polymerase I.
Catalogue NumberPF059
Brand Family Calbiochem®
References
ReferencesTelford, J.L. et al. 1979. Proc. Natl. Acad. Sci. USA 76, 2590.
Wickens, M.P. 1978. J. Biol. Chem. 253, 2483.
Sanger, F., et al. 1977. Proc. Natl. Acad. Sci. USA 74, 5463.
Jaccobsen, H., et al. 1974. Eur. J. Biochem. 45, 623.
Product Information
Unit of DefinitionOne unit is the amount of enzyme required to incorporate 10 nmol of total nucleotide into acid-insoluble form in 30 min at 37°C.
FormLiquid
FormulationIn 50 mM potassium phosphate buffer, 0.25 mM dithiothreitol, 50% glycerol, pH 7.0.
PreservativeNone
Applications
Application NotesDNA Labeling
Application CommentsOne unit is the amount of enzyme required to incorporate 10 nmol of total nucleotide into acid-insoluble form in 30 minutes at 37°C.
Biological Information
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
PF059 0

Documentation

Klenow Enzyme MSDS

Title

Safety Data Sheet (SDS) 

Klenow Enzyme Certificates of Analysis

TitleLot Number
PF059

References

Přehled odkazů
Telford, J.L. et al. 1979. Proc. Natl. Acad. Sci. USA 76, 2590.
Wickens, M.P. 1978. J. Biol. Chem. 253, 2483.
Sanger, F., et al. 1977. Proc. Natl. Acad. Sci. USA 74, 5463.
Jaccobsen, H., et al. 1974. Eur. J. Biochem. 45, 623.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision16-September-2008 RFH
ApplicationDNA Labeling
DescriptionKlenow Enzyme is the large fragment of DNA polymerase I which can be produced by cleavage of intact DNA Polymerase I with subtilisin. The Klenow fragment exhibits 5’ → 3’ DNA polymerase and 3' → 5' exonuclease activity, but lacks the 5’ → 3’ exonuclease activity of intact DNA polymerase I. Klenow is used to fill in recessed 3’ termini as in end-labeling or in the synthesis of the second strand of cDNA from a single-stranded template. Klenow is also used in dideoxy sequencing reactions.
BackgroundKlenow Enzyme is the large fragment of DNA polymerase I which can be produced by cleavage of intact DNA Polymerase I with subtilisin. The Klenow fragment exhibits 5’ → 3’ DNA polymerase and 3' → 5' exonuclease activity, but lacks the 5’ → 3’ exonuclease activity of intact DNA polymerase I. Klenow is used to fill in recessed 3’ termini as in end-labeling or in the synthesis of the second strand of cDNA from a single-stranded template. Klenow is also used in dideoxy sequencing reactions.
FormLiquid
FormulationIn 50 mM potassium phosphate buffer, 0.25 mM dithiothreitol, 50% glycerol, pH 7.0.
Concentration Label Please refer to vial label for lot-specific concentration
Unit definitionOne unit is the amount of enzyme required to incorporate 10 nmol of total nucleotide into acid-insoluble form in 30 min at 37°C.
SolubilityFollowing initial thaw, aliquot and freeze (-20°C).
PreservativeNone
CommentsOne unit is the amount of enzyme required to incorporate 10 nmol of total nucleotide into acid-insoluble form in 30 minutes at 37°C.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesTelford, J.L. et al. 1979. Proc. Natl. Acad. Sci. USA 76, 2590.
Wickens, M.P. 1978. J. Biol. Chem. 253, 2483.
Sanger, F., et al. 1977. Proc. Natl. Acad. Sci. USA 74, 5463.
Jaccobsen, H., et al. 1974. Eur. J. Biochem. 45, 623.