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431050 InSolution™ Leptomycin B, Streptomyces sp. - CAS 87081-35-4 - Calbiochem

InSolution™ Leptomycin B, Streptomyces sp., CAS 87081-35-4, is a 20 µg/ml solution in ethanol. An antifungal agent that blocks nuclear export by impairing the function of nuclear export factor CRM1.

InSolution™ Leptomycin B, Streptomyces sp. - CAS 87081-35-4 - Calbiochem MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: LMB, Chromosome Region Maintenance 1 Protein Inhibitor I, CRM1 Inhibitor I, Exportin 1 Inhibitor I, XPO1 Inhibitor I

Primary Target: HIV-1 replication in primary human monocytes
431050
  
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Přehled

Replacement Information

Tabulka spec. kláve

CAS #Empirical Formula
87081-35-4C₃₃H₄₈O₆
Description
OverviewAn antifungal antibiotic that acts as an inhibitor of nuclear export because of its ability to interact with and impair the function of the nuclear export factor CRM1. It is about twice as potent as Leptomycin A (Cat. No. 431051) in inhibiting nucleocytoplasmic translocation of Rev, blocks the Rev-dependent export of mRNA into cytoplasm, and suppresses HIV-1 replication in primary human monocytes (IC50 = 600 pM). In addition, LMB effectively induces the accumulation of p53 in the nucleus of normal fibroblasts as well as protects p53 from Mdm2-mediated ubiquitination and degradation.
Caution! Product contains volatile liquid. Keep product cold at all times.
Catalogue Number431050
Brand Family Calbiochem®
SynonymsLMB, Chromosome Region Maintenance 1 Protein Inhibitor I, CRM1 Inhibitor I, Exportin 1 Inhibitor I, XPO1 Inhibitor I
References
ReferencesKau, T.R., and Silver, P.A. 2003. Drug Discov. Today 8, 78.
Menendez, S., et al. 2003. Br. J. Cancer 88, 636.
Xirodimas, D.P., et al. 2001. Exp. Cell Res. 270, 66.
Kudo, N., et al. 1999. Proc. Natl. Acad. Sci. USA 96, 9112.
Lain, S., et al. 1999. Exp. Cell Res. 248, 457.
Wolff, B., et al. 1997. Chem. Biol. 4, 139.
Yoshida, M., et al. 1990. Exp. Cell Res. 187, 150.
Komiyama, K., et al. 1985. J. Antibiot. 38, 427.
Product Information
CAS number87081-35-4
ATP CompetitiveN
FormLiquid
FormulationSupplied at 20 µg/ml (5 µg in 250 µl) in absolute ethanol
Hill FormulaC₃₃H₄₈O₆
Chemical formulaC₃₃H₄₈O₆
ReversibleN
Structure formula ImageStructure formula Image
Quality LevelMQ100
Applications
ApplicationInSolution™ Leptomycin B, Streptomyces sp., CAS 87081-35-4, is a 20 µg/ml solution in ethanol. An antifungal agent that blocks nuclear export by impairing the function of nuclear export factor CRM1.
Biological Information
Primary TargetHIV-1 replication in primary human monocytes
Primary Target IC<sub>50</sub>600 pM in suppressing HIV-1 replication in primary human monocytes
Purity≥95% by HPLC
Physicochemical Information
Cell permeableN
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Flammable
Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Storage ≤ -70°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsFollowing initial use, aliquot and freeze (-70°C).
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
431050 0

Documentation

InSolution™ Leptomycin B, Streptomyces sp. - CAS 87081-35-4 - Calbiochem MSDS

Title

Safety Data Sheet (SDS) 

InSolution™ Leptomycin B, Streptomyces sp. - CAS 87081-35-4 - Calbiochem Certificates of Analysis

TitleLot Number
431050

References

Přehled odkazů
Kau, T.R., and Silver, P.A. 2003. Drug Discov. Today 8, 78.
Menendez, S., et al. 2003. Br. J. Cancer 88, 636.
Xirodimas, D.P., et al. 2001. Exp. Cell Res. 270, 66.
Kudo, N., et al. 1999. Proc. Natl. Acad. Sci. USA 96, 9112.
Lain, S., et al. 1999. Exp. Cell Res. 248, 457.
Wolff, B., et al. 1997. Chem. Biol. 4, 139.
Yoshida, M., et al. 1990. Exp. Cell Res. 187, 150.
Komiyama, K., et al. 1985. J. Antibiot. 38, 427.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision28-September-2007 RFH
SynonymsLMB, Chromosome Region Maintenance 1 Protein Inhibitor I, CRM1 Inhibitor I, Exportin 1 Inhibitor I, XPO1 Inhibitor I
DescriptionAn unsaturated, branched-chain fatty acid with a terminal lactone ring that acts as a potent inhibitor of CRM-1-mediated (exportin-1) nuclear export. Exhibits antibiotic, anti-fungal, and anti-tumor properties and blocks the progression of the cell cycle at the G1 and G2 phases. Shown to inhibit nucleo-cytoplasmic translocation of Rev at nanomolar concentrations and block Rev-dependent export of mRNA into the cytoplasm. Also shown to suppress HIV-1 replication in primary human monocytes (IC50 = 600 pM). Effectively induces the accumulation of p53 in the nucleus of normal fibroblasts and protects p53 from Mdm2-mediated ubiquitination and degradation. Useful for studies involving nuclear localization and protein trafficking in eukaryotic cells.

Caution! Product contains volatile liquid. Keep product cold at all times.
FormLiquid
FormulationSupplied at 20 µg/ml (5 µg in 250 µl) in absolute ethanol
Intert gas (Yes/No) Packaged under inert gas
CAS number87081-35-4
Chemical formulaC₃₃H₄₈O₆
Structure formulaStructure formula
Purity≥95% by HPLC
Storage Protect from light
≤ -70°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial use, aliquot and freeze (-70°C).
Toxicity Flammable
ReferencesKau, T.R., and Silver, P.A. 2003. Drug Discov. Today 8, 78.
Menendez, S., et al. 2003. Br. J. Cancer 88, 636.
Xirodimas, D.P., et al. 2001. Exp. Cell Res. 270, 66.
Kudo, N., et al. 1999. Proc. Natl. Acad. Sci. USA 96, 9112.
Lain, S., et al. 1999. Exp. Cell Res. 248, 457.
Wolff, B., et al. 1997. Chem. Biol. 4, 139.
Yoshida, M., et al. 1990. Exp. Cell Res. 187, 150.
Komiyama, K., et al. 1985. J. Antibiot. 38, 427.