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IPVH10100 Immobilon®-P PVDF Membrane

IPVH10100
10  
Purchase on Sigma-Aldrich

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Replacement Information
Replacement InformationINCP 101 00 (Protein Applications)

Tabulka spec. kláve

Key Applications
WB, DB
Description
Catalogue NumberIPVH10100
ReplacesINCP10100
Trade Name
  • Immobilon
DescriptionImmobilon®-P PVDF Membrane
Background InformationOverview
- Millipore offers four different Immobilon PVDF membranes, each optimized for different protein blotting applications.
Immobilon PVDF membranes provide many advantages compared to nitrocellulose. They won’t crack or curl, and they can be cut without fracturing. They also have low background, broad solvent compatibility, and superior staining capabilities. In addition, they can be reprobed multiple times.

Immobilon-P Transfer Membrane:
- 45 µm pore size Recommended for most western blotting, especially proteins greater than 20 kDa
- Millipore's Rapid Immunodetection Protocol reduces detection times by up to 2 hours by eliminating membrane blocking and several wash steps with no loss of sensitivity or specificity

Immobilon-E Membrane:
- the only PVDF membrane that wets out in aqueous buffers, eliminating the methanol pre-wet step.
- 45 µm pore size recommended for most western blotting, especially proteins greater than 20 kDa

Blotting Sandwiches:
- When you need to process multiple blots, blotting sandwiches are a convenient, time-saving choice. Our sandwiches include sheets of Immobilon-P membrane interleaved with pre-cut sheets of chromatography-grade blotting filter paper and are compatible with any standard wet transfer device.

Blotting Filter Paper:
- Chromatography-grade blotting filter paper pre-cut to the most popular western blotting sizes.

Immobilon-PSQ Membrane:
- 0.2 µm pore size and a large internal structure
- Higher protein adsorption and sequencing yields than other membranes
- Recommended for blotting proteins less than 20 kDa
- Prevents blow-through of low molecular weight proteins

Immobilon-FL Membrane:
- The first transfer membrane optimized for fluorescence applications
- Extremely low background improves sensitivity of all fluorescence detection protocols
- Compatible with all commonly used fluorescent probes at all excitation and emission wavelengths
- Ideal for multiplexing and chemifluorescence applications

Immobilon® PVDF membranes are offered in four types, each optimized for a different protein blotting application. Convenient blotting sandwiches feature pre-cut sheets of membrane and blotting filter paper. Immobilon® PVDF membranes have high protein adsorption, so you won’t lose proteins during transfer or reprobing. The open pore structure makes it easy to access bound proteins and remove unbound probes. In addition, Immobilon® PVDF membranes optimized for fluorescent blots dramatically increase signal-to-noise ratios for high sensitivity in quantitative, multiplexing applications.

Features & Benefits:
- Won’t crack, curl or fracture when cut
- Low background
- Superior staining capabilities
- Can be reprobed multiple times

Applications:
- Western Blotting, Immunoblotting, Dot Blotting, Protein Sequencing;
- Compatible with Chemiluminescent, Fluorescent, Radioactive, Chromogenic, and Chemifluorescent Detection
- Compatible with all commonly used fluorescent probes at all excitation and emission wavelengths
References
Product Information
Detection methodChemiluminescence Chromogenic Radioactive
HS Code3921 19 00
Compatible Stains
  • Coomassie brilliant blue
  • Amido black
  • India ink
  • Ponceau-S red
  • Colloidal gold
  • CPTS
  • Toluidine blue
  • Transillumination
  • Sypro® ruby
Filter CodeIPVH
Filter ColorWhite
Quality LevelMQ400
Applications
Application10 sheets, 10 cm x 10 cm, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane for western blotting.
Key Applications
  • Western Blotting
  • Dot Blot
Biological Information
Primary Binding MechanismsElectrostatic, hydrophobic
BSA Adsorption215 µg/cm²
Goat IgG Adsorption294 µg/cm²
Insulin Adsorption160 µg/cm²
WettabilityHydrophobic
Physicochemical Information
Pore Size0.45 µm
Dimensions
Filter SurfacePlain
Filter Dimensions10 cm x 10 cm
Materials Information
Chemistry
  • Hydrophobic Polyvinylidene Fluoride (PVDF)
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Packaging Information
Material Size10
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
IPVH10100 08436037125751

Documentation

Immobilon®-P PVDF Membrane MSDS

Title

Safety Data Sheet (SDS) 

References

Reference overviewApplicationPub Med ID
Identification of target antigens of anti-endothelial cell and anti-vascular smooth muscle cell antibodies in patients with giant cell arteritis: a proteomic approach.
Régent, Alexis, et al.
Arthritis research & therapy, 13: R107 (2011)  2010

Zobrazit abstrakt
21711540 21711540
A role for a CXCR2/phosphatidylinositol 3-kinase gamma signaling axis in acute and chronic vascular permeability.
Gavard, Julie, et al.
Mol. Cell. Biol., 29: 2469-80 (2009)  2009

Zobrazit abstrakt
Immunoblotting (Western)19255141 19255141
Quantitation of Protein on gels and blots by infrared fluorescence of Coomassie blue and fast green
Luo S., Wehr N.B., Levine R.L.
Analytical Biochemistry:350 (2006):233-238  2005

Immunoblotting (Western)
Biochemical analysis of GABA receptor subunits alpha 1, alpha 5, beta1 beta2 in the hippocampus of patients with Alzheimer's disease neurophathology
Rissman, R.A., Mishizen-Eberz A.J. N.,Wolfe, C.B.B., DeBlas A.L., Miralles C.P., Ikonomovic M.D., armstrong D.M.
Neuroscience 120 (2003) 295-705  2003

Western Blotting

Brochure

Title
Western Blotting Tools

Technical Info

Title
Protein Blotting Handbook: 6th Edition

Data Sheet

Title
Immobilon Transfer Membranes: For superior protein and nucleic acid blots

User Guides

Title
Immobilon -P Blotting Sandwiches
Immobilon®-P Transfer Membrane User Guide
User Guide - Immobilon®P Transfer Membrane