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71867 Gateway® Nova pCOLA-2-DEST Expression System

Gateway® Nova pCOLA-2-DEST Expression System MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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Description
Overview

This product has been discontinued.





The Gateway® Nova pCOLA-2-DEST™ vector carries the COLA replicon from ColA and the kanamycin resistance gene. This DEST vector is compatible with pENTR vectors created using pDONR223, the pDONR vector used in the human ORFeome project. This DEST vector is NOT compatible with pENTR vectors derived from pDONR221 or other pENTR vectors carrying the kanR gene on their backbone. pEXPR recombinants created using pCOLA-2-DEST can be used for coexpression with expression plasmids generated from Gateway Nova pET-DEST vectors carrying the ColE1 replicon and AmpR gene. pCOLA-2-DEST is designed to create expression clones (pEXPR) that are fused to an N-terminal His•Tag® coding sequence. If the pENTR clone lacks a stop codon and is appropriately designed for a C-terminal fusion, the target gene in the resulting pEXPR clone will also be fused to a C-terminal Strep•Tag® II coding sequence. The expression system contains Nova F- Competent Cells for cloning, Rosetta™ 2 (DE3) Competent Cells for expression, control plasmids for transformation, recombination & expression, and the LR Clonase® II enzyme for the recombination reaction.
Catalogue Number71867
Brand Family Novagen®
References
Product Information
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
R PhraseR: 36/38

Irritating to eyes and skin.
S PhraseS: 22-26-36

Do not breathe dust.
In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing.
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Irritant
Storage ≤ -70°C
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
71867 0