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14-306 Sigma-AldrichGSK3β Protein, active, 10 µg

Active, N-terminal His6-tagged recombinant human GSK3β with an H350L mutation, for use in Kinase Assays.

GSK3β Protein, active, 10 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

SDS
CoA
References
FAQs

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Description
Catalogue Number	14-306
Brand Family	Upstate
Trade Name	Upstate
Description	GSK3β Protein, active, 10 µg
Overview	N-terminal His6-tagged recombinant human GSK3β with an H350L mutation

References

Product Information
Quality Level	MQ100

Applications
Application	Active, N-terminal His6-tagged recombinant human GSK3β with an H350L mutation, for use in Kinase Assays.
Key Applications	Kinase Assay

Biological Information
Source	Expressed by baculovirus in Sf21 insect cells
Specific Activity	For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number	NM_002093.2
Entrez Gene Summary	Glycogen synthase kinase-3 (GSK3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase. Two isoforms, alpha (GSK3A; MIM 606784) and beta, show a high degree of amino acid homology (Stambolic and Woodgett, 1994 [PubMed 7980435]). GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation (Plyte et al., 1992 [PubMed 1333807]).[supplied by OMIM]
Gene Symbol	GSK3B
Protein Target	GSK3β
Purification Method	Ni²⁺/NTA-agarose
Target Sub-Family	CMGC
UniProt Number	P49841
UniProt Summary	FUNCTION: SwissProt: P49841 # Participates in the Wnt signaling pathway. Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. SIZE: 420 amino acids; 46744 Da SUBUNIT: Monomer (By similarity). Interacts with NIN and CABYR. TISSUE SPECIFICITY: Expressed in testis, thymus, prostate and ovary and weakly expressed in lung, brain and kidney. PTM: Phosphorylated by AKT1 and ILK1. SIMILARITY: SwissProt: P49841 ## Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily. & Contains 1 protein kinase domain.
Molecular Weight	51kDa

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Routinely evaluated by phosphorylation of Phospho-Glycogen Synthase Peptide-2
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	6 months at -70°C

Packaging Information
Material Size	10 µg
Material Package	Also available in 250μg size (2x125μg)--call for pricing and availability and reference catalog number 14-306M when ordering the 250μg size.

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Katalogové číslo	GTIN
14-306	04053252409486

Documentation

GSK3β Protein, active, 10 µg MSDS

Title
Safety Data Sheet (SDS)

GSK3β Protein, active, 10 µg Certificates of Analysis

Title	Lot Number
GSK-3#946;, active - D8JN013U-T	D8JN013U-T
GSK-3#946;, active - D8JN013U-V	D8JN013U-V
GSK-3#946;, active - D8JN013U-W	D8JN013U-W
GSK-3#946;, active - D8JN013U-X	D8JN013U-X
GSK-3#946;, active - D8JN013U-Y	D8JN013U-Y
GSK-3#946;, active - D8JN013U-Z	D8JN013U-Z
GSK-3ß, active - D8JN013UAA	D8JN013UAA
GSK-3β, active - D8JN013FU	D8JN013FU
GSK-3β, active - WAA0024-I	WAA0024-I
GSK‐3β ,active - D8JN013UA-A	D8JN013UA-A

References

Reference overview	Application	Pub Med ID
Ethanol impairs insulin-stimulated neuronal survival in the developing brain: role of PTEN phosphatase Xu, J., et al J Biol Chem, 278:26929-37 (2003) 2003	Kinase Assay	12700235
Protein kinase involved in lung injury susceptibility: Evidence from enzyme isoform genetic knockout and in vivo inhibitor treatment Wainwright, M. S., et al Proc Natl Acad Sci U S A, 100:6233-8 (2003) 2003	Activation Assay	12730364
The alpha-isoform of glycogen synthase kinase-3 from rabbit skeletal muscle is inactivated by p70 S6 kinase or MAP kinase-activated protein kinase-1 in vitro. Sutherland, C and Cohen, P FEBS Lett., 338: 37-42 (1994) 1993 Zobrazit abstrakt The alpha-isoform of glycogen synthase kinase-3 (GSK3 alpha) was inactivated by 80% towards a synthetic peptide substrate upon incubation with Mg-ATP and either MAP kinase-activated protein (MAPKAP) kinase-1 or p70 S6 kinase. Inactivation by either kinase resulted from the phosphorylation of Ser-21 and was reversed by treatment with protein phosphatase 2A1. Phosphorylation also decreased GSK3 alpha activity towards glycogen synthase, inhibitor-2 and c-jun. The specificity of GSK3 alpha was similar to GSK3 beta, but with the synthetic peptide substrate heparin stimulated the dephosphorylated form of GSK3 alpha (6-fold) more than GSK3 beta (1.8-fold). After phosphorylation, both isoforms were stimulated 15-20-fold by heparin.		8307153
Regulation and functions of the glycogen synthase kinase-3 subfamily. Woodgett, J R Semin. Cancer Biol., 5: 269-75 (1994) 1993 Zobrazit abstrakt Glycogen synthase kinase-3 is a 'black sheep' among protein kinases. Although its name suggests a primary function in intermediary metabolism, its scope is far more diverse as indicated by both genetic and biochemical evidence. Over the past five years the enzyme has emerged as an important component of transduction pathways ultimately regulating cell fate and differentiation. The molecular mechanisms underlying these functions are only just being unraveled but point to a novel mode of cellular regulation conserved in all eukaryotes.		7803763

FAQ

Question	Answer
Why is it important to shake the reaction mixture during the incubations?	It is important to shake the reaction mixture during incubations so that the substrate and the enzyme get good interaction. If you do not have access to a shaking incubator, use a water bath for the incubations, removing the tubes regularly to vortex.

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Life Science Research > Proteins and Enzymes > Purified Kinases

Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases

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