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341589 FGF, Acidic, Mouse, Recombinant

FGF, Acidic, Mouse, Recombinant MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: HBGF-1

341589
  
Purchase on Sigma-Aldrich

Přehled

Replacement Information

Tabulka spec. kláve

Purity
≥95% SDS-PAGE
Description
Overview

This product has been discontinued.



Recombinant, mouse fibroblast growth factor (FGF) acidic, consisting of amino acids 16-155 and expressed in E. coli, with an N-terminal Met. FGF acidic stimulates proliferation of all cells of mesodermal origin and many cells of ectodermal, neuroectodermal, and endodermal origin. Intracellular FGF acidic inhibits p53 activity and proapoptotic signaling, thereby enabling it to function as a survival factor.
Catalogue Number341589
Brand Family Calbiochem®
SynonymsHBGF-1
References
ReferencesGuerrini, M., et al. 2007. Curr. Pharm. Des. 13, 2045.
Nilsen, T., et al. 2007. J. Biol. Chem. 282, 26245.
Rajalingam, D., et al. 2007. Biochemistry 46, 9225.
Wesche, J., et al. 2006. J. Biol. Chem. 281, 11405.
Bouleau, S., et al. 2005. Oncogene 24, 7839.
Mohammadi, M., et al. 2005. Cytokine Growth Factor Rev. 16, 107.
Presta, M., et al. 2005. Cytokine Growth Factor Rev. 16, 159.
Robinson, C.J., et al. 2005. J. Biol. Chem. 280, 42274.
Wesche, J., et al. 2005. Biochemistry 44, 6071.
Wiedlocha, A., et al. 2005. Mol. Biol. Cell 16, 794.
Wiedlocha, A. and V. Sorensen. 2004. Curr. Top. Microbiol. Immunol. 286, 45.
Allen, B.L. and A.C. Rapraeger. 2003. J. Cell Biol. 163, 637.
Galzie, Z., et al. 1997. Biochem. Cell Biol. 75, 669.
Madiai, F., et al. 1996. Gene 179, 231.
Imamura, T., et al. 1990. Science 249, 1567.
Product Information
FormSolid
FormulationLyophilized from 0.2 µm sterile-filtered solution in MOPS, Na₂SO₄, EDTA, DTT, BSA.
Quality LevelMQ100
Applications
Biological Information
Biological activityED₅₀ = 0.05-0.25 ng/ml, in the presence of 10 µg/ml heparin, as measured in a cell proliferation assay using NR6R-3T3 mouse fibroblasts.
Purity≥95% SDS-PAGE
Physicochemical Information
ContaminantsEndotoxin: <1.0 EU/µg protein
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
341589 0

Documentation

FGF, Acidic, Mouse, Recombinant MSDS

Title

Safety Data Sheet (SDS) 

FGF, Acidic, Mouse, Recombinant Certificates of Analysis

TitleLot Number
341589

References

Přehled odkazů
Guerrini, M., et al. 2007. Curr. Pharm. Des. 13, 2045.
Nilsen, T., et al. 2007. J. Biol. Chem. 282, 26245.
Rajalingam, D., et al. 2007. Biochemistry 46, 9225.
Wesche, J., et al. 2006. J. Biol. Chem. 281, 11405.
Bouleau, S., et al. 2005. Oncogene 24, 7839.
Mohammadi, M., et al. 2005. Cytokine Growth Factor Rev. 16, 107.
Presta, M., et al. 2005. Cytokine Growth Factor Rev. 16, 159.
Robinson, C.J., et al. 2005. J. Biol. Chem. 280, 42274.
Wesche, J., et al. 2005. Biochemistry 44, 6071.
Wiedlocha, A., et al. 2005. Mol. Biol. Cell 16, 794.
Wiedlocha, A. and V. Sorensen. 2004. Curr. Top. Microbiol. Immunol. 286, 45.
Allen, B.L. and A.C. Rapraeger. 2003. J. Cell Biol. 163, 637.
Galzie, Z., et al. 1997. Biochem. Cell Biol. 75, 669.
Madiai, F., et al. 1996. Gene 179, 231.
Imamura, T., et al. 1990. Science 249, 1567.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision21-Sepmtember-2011 RFH
SynonymsHBGF-1
DescriptionRecombinant, mouse fibroblast growth factor (FGF) acidic, consisting of amino acids 16-155 and expressed in E. coli, with an N-terminal Met. FGF acidic stimulates proliferation of all cells of mesodermal origin and many cells of ectodermal, neuroectodermal, and endodermal origin. Intracellular FGF acidic inhibits p53 activity and proapoptotic signaling, thereby enabling it to function as a survival factor.
FormSolid
FormulationLyophilized from 0.2 µm sterile-filtered solution in MOPS, Na₂SO₄, EDTA, DTT, BSA.
Purity≥95% SDS-PAGE
ContaminantsEndotoxin: <1.0 EU/µg protein
Biological activityED₅₀ = 0.05-0.25 ng/ml, in the presence of 10 µg/ml heparin, as measured in a cell proliferation assay using NR6R-3T3 mouse fibroblasts.
SolubilityReconstitute to 100 µg/ml in sterile PBS containing at least 0.1% human or bovine serum albumin.
Storage -20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
Toxicity Standard Handling
ReferencesGuerrini, M., et al. 2007. Curr. Pharm. Des. 13, 2045.
Nilsen, T., et al. 2007. J. Biol. Chem. 282, 26245.
Rajalingam, D., et al. 2007. Biochemistry 46, 9225.
Wesche, J., et al. 2006. J. Biol. Chem. 281, 11405.
Bouleau, S., et al. 2005. Oncogene 24, 7839.
Mohammadi, M., et al. 2005. Cytokine Growth Factor Rev. 16, 107.
Presta, M., et al. 2005. Cytokine Growth Factor Rev. 16, 159.
Robinson, C.J., et al. 2005. J. Biol. Chem. 280, 42274.
Wesche, J., et al. 2005. Biochemistry 44, 6071.
Wiedlocha, A., et al. 2005. Mol. Biol. Cell 16, 794.
Wiedlocha, A. and V. Sorensen. 2004. Curr. Top. Microbiol. Immunol. 286, 45.
Allen, B.L. and A.C. Rapraeger. 2003. J. Cell Biol. 163, 637.
Galzie, Z., et al. 1997. Biochem. Cell Biol. 75, 669.
Madiai, F., et al. 1996. Gene 179, 231.
Imamura, T., et al. 1990. Science 249, 1567.