Pokud jste položku nepřidali do nákupního košíku nebo do oblíbených, vaše konfigurace se při zavření neuloží.
Chcete-li zavřít nástroj MILLIPLEX® MAP, klikněte na OK. Pokud se chcete vrátit ke svým výběrům, klikněte na Zrušit.
Vyberte přizpůsobitelné panely a soupravy smíchané předem – NEBO - soupravy buněčné signalizace MAPmates™
Navrhněte si a oceňte své soupravy MILLIPLEX® MAP.
Přizpůsobitelné panely a soupravy smíchané předem
Naše široké portfolio se skládá z multiplexních panelů, které vám umožňují vybrat v rámci panelu analyty přesně podle vašich představ. Na zvláštní kartě si můžete zvolit formát předem smíchaných cytokinů, nebo soupravu single plex.
Soupravy buněčné signalizace a MAPmates™
Vyberte pevné soupravy, které vám umožní prozkoumat celé cesty nebo procesy. Nebo si sestavte své vlastní soupravy ze single plex MAPmates™, při dodržení uvedených pokynů.
Následující MAPmates™ by se neměly spolu mísit: -MAPmates™ vyžadující jiný testovací pufr -Fosfo-specifické a celkové páry MAPmate™, např. celkové GSK3β a GSK3β (Ser 9) -PanTyr a MAPmates™ specifické pro lokalitu, např. receptor fosfo-EGF a fosfo-STAT1 (Tyr701) -Více než 1 fosfo-MAPmate™ pro jediný cíl (Akt, STAT3) -GAPDH a β-Tubulin nelze mísit se soupravami nebo MAPmates™ obsahujícími panTyr
.
Katalogové číslo
Popis objednávky
ks/bal.
Seznam
Položka byla přidána do oblíbených.
Vyberte druh, typ panelu, soupravu nebo typ vzorku
Chcete-li začít s návrhem vaší soupravy MILLIPLEX® MAP, vyberte příslušný druh, typ panelu nebo soupravu.
Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
Catalogue Number
Ordering Description
Qty/Pack
List
Položka byla přidána do oblíbených.
Druh
Typ panelu
Vybraná souprava
ks
Katalogové číslo
Popis objednávky
ks/bal.
Cena podle ceníku
96-Well Plate
ks
Katalogové číslo
Popis objednávky
ks/bal.
Cena podle ceníku
Přidat další reagencie (Pro použití s MAPmates jsou vyžadovány pufr a detekční souprava)
ks
Katalogové číslo
Popis objednávky
ks/bal.
Cena podle ceníku
48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
Možnost úspory místa Zákazníci, kteří si pořizují různé soupravy, se mohou rozhodnout pro úsporu místa tím, že nevyžadují balení soupravy a zboží si nechají dodat ve formě multiplexních komponent testů v plastových sáčcích, které se pak kompaktněji skladují.
Položka byla přidána do oblíbených.
Produkt byl přidán do vašeho nákupního košíku
Nyní můžete upravit další soupravu, vybrat předem smíchanou soupravu, odhlásit se nebo zavřít objednací nástroj.
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331569
Sigma-AldrichEthidium Bromide Adsorber - CAS 69011-20-7 - Calbiochem
Developed specifically for the safe and simple removal of ethidium bromide (EtBr) from aqueous staining solutions and running buffers used in nucleic acid separation gels.
More>>Developed specifically for the safe and simple removal of ethidium bromide (EtBr) from aqueous staining solutions and running buffers used in nucleic acid separation gels. Less<<
Ethidium Bromide Adsorber - CAS 69011-20-7 - Calbiochem MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Developed specifically for the safe and simple removal of ethidium bromide (EtBr) from aqueous staining solutions and running buffers used in nucleic acid separation gels. Typical concentrations in these solutions are 0.5 - 10 mg/L. EtBr is bound and concentrated by the adsorber column, allowing removal of the filtrate. Each column normally binds at least 300 mg of ethidium bromide from TAE or TBE buffers. Residual capacity is easily visualized because the EtBr appears as a dark red to black band on the column. As long as this band has not reached the bottom of the column bed, the concentration of dye is still below the column capacity.
Catalogue Number
331569
Brand Family
Calbiochem®
Application Data
In order to begin flow, the two plug caps must be removed from the adaptors. These are tightly fit and may require some effort to remove them.
References
References
MacGregor, J.T., and Johnson, I.J. 1977. Mutat. Res.48, 103. Waring, M. 1975. In: Antibiotics, Corcoran, J.W., and Hahn, F.E., Editors, Vol. 3, 141, Cold Spring Harbor, NY.
Removal of ethidium bromide (EtBr) from aqueous staining solutions and running buffers
Physicochemical Information
Cell permeable
N
Safety Information
R Phrase
R: 36
Irritating to eyes.
Storage and Shipping Information
Ship Code
Ambient Temperature Only
Toxicity
Irritant
Storage
+15°C to +30°C
Do not freeze
Ok to freeze
Special Instructions
When the adsorber column is not in use, both ends should be closed off. The column should not be allowed to dry out, as air bubbles may form in the column, hindering flow rate.
Global Trade Item Number
Katalogové číslo
GTIN
1.02243.0001
04022536510033
331569-1EA
04055977195750
Documentation
Ethidium Bromide Adsorber - CAS 69011-20-7 - Calbiochem MSDS
Ethidium Bromide Adsorber - CAS 69011-20-7 - Calbiochem Certificates of Analysis
Title
Lot Number
331569
References
Přehled odkazů
MacGregor, J.T., and Johnson, I.J. 1977. Mutat. Res.48, 103. Waring, M. 1975. In: Antibiotics, Corcoran, J.W., and Hahn, F.E., Editors, Vol. 3, 141, Cold Spring Harbor, NY.
Data Sheet
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
16-December-2010 RFH
Application Data
In order to begin flow, the two plug caps must be removed from the adaptors. These are tightly fit and may require some effort to remove them.
Description
Developed specifically for the safe and simple removal of ethidium bromide (EtBr) from aqueous staining solutions and running buffers used in nucleic acid separation gels. Typical concentrations in these solutions are 0.5-10 mg/l. EtBr is bound and concentrated by the adsorber column, allowing removal of the filtrate. Each column normally binds at least 300 mg of ethidium bromide from TAE or TBE buffers. Residual capacity is easily visualized because the EtBr appears as a dark red to black band on the column. As long as this band has not reached the bottom of the column bed, the concentration of dye is still below the column capacity.
Form
Gray-brown suspension
Recommended reaction conditions
Ethidium bromide (EtBr) is the fluorescence dye most frequently used to stain nucleic acids in agarose and polyacrylamide gels. It intercalates primarily with double-stranded DNA to form an orange-red fluorescent complex under the influence of UV light. This interaction with nucleic acids makes ethidium bromide potentially mutagenic and is classified as toxic.
Capacity
The column capacity is at least 300 mg EtBr, corresponding to 30 L gel staining solution. The binding capacity of the cartridge is tested with a 10 mg/L EtBr solution of 1X TAE buffer; >99% of the dye was adsorbed up to the 300 mg limit as measured by fluorescence spectrometry.
Flow Rate
The flow rate of the adsorber column depends on the volume and height of the reservoir used for the staining solution and is a minimum of 200 ml/hr.
Connecting the Adsorber Column
The cartridge, as supplied, is sealed at both ends with small plastic caps (see figure 1). These caps should be removed prior to use and the cartridge connected to a reservoir containing the ethidium bromide solution to be adsorbed. A glass or plastic reservoir fitted with a stopcock or tubing clamp should be used to that the column can be switched on and off as required. To avoid any blocking of the frits with gel particles in the staining solutions, it is advisable to put some glass wool, filter paper, etc. in the connecting tube. If the flow rate of the adsorber column is distinctly lower than 1-2 drops per second, residual air bubbles must be removed by repeatedly short-squeezing the tubing above the cartridge. To guarantee a good flow rate throughout the lifespan of the cartridge, the formation of air bubbles in the system should be avoided, as these tend to hinder flow. The residual capacity of the column is easy to assess visually as the ethidium bromide moves through the column in the form of an increasingly dark red to black band. As long as this band has not reached the bottom of the column, the concentration of dye is still below the specified detection limit.
Figure 1: Ethidium Bromide Adsorber
In order to begin flow, the two plug caps must be removed from the adaptors. These are tightly fit and may require some effort to remove them.
Disposal
Once the capacity has been exhausted, the adsorber column should be disposed of according to local regulations.
CAS number
69011-20-7
Storage
+15°C to +30°C
Do Not Freeze
Ok to freeze
Special Instructions
When the adsorber column is not in use, both ends should be closed off. The column should not be allowed to dry out, as air bubbles may form in the column, hindering flow rate.
Toxicity
Irritant
References
MacGregor, J.T., and Johnson, I.J. 1977. Mutat. Res.48, 103. Waring, M. 1975. In: Antibiotics, Corcoran, J.W., and Hahn, F.E., Editors, Vol. 3, 141, Cold Spring Harbor, NY.