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2384 Creatine Phosphokinase, Rabbit Skeletal Muscle

2384
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Katalogové čísloBalení ks/bal.
2384-20MG Plastová ampulka 20 mg
Description
OverviewNative creatine phosphokinase from rabbit muscle. Catalyzes the conversion of creatine and ATP to creatine phosphate and ADP. Has an optimal pH of 9.0 for the forward reaction and 6.0-7.0 for the reverse reaction.
Catalogue Number2384
Brand Family Calbiochem®
SynonymsrCPK
References
ReferencesBessman, S.P. 1987. Anal. Biochem. 161, 519.
Product Information
CAS number9001-15-4
Activity≥300 units/mg (at 25°C); 750 units/mg (at 37°C)
Unit of DefinitionOne unit is defined as the amount of enzyme that will catalyze the conversion of 1.0 µmol creatine phosphate per min at 25°C or 37°C, pH 6.8.
EC number2.7.3.2
FormLyophilized
Hygroscopic Hygroscopic
Quality LevelMQ200
Physicochemical Information
ContaminantsATPase, hexokinase, myokinase: <0.001%
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Hygroscopic Hygroscopic
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution in 0.5% NaHCO₃, refrigerate (4°C). Do not freeze or shake, as SH groups may be oxidized and form linkages between protein molecules, resulting in precipitation, turbidity, and loss of activity. NaHCO₃ stock solutions are stable for 2-3 days at 4°C. Following reconstitution in 0.1 M imidazole buffer, aliquot and freeze (-20°C). Imidazole stock solutions are stable for up to 4 weeks at -20°C. Avoid freeze/thaw cycles, as 20-30% of activity will be lost with each freeze/thaw cycle.
Global Trade Item Number
Katalogové číslo GTIN
2384-20MG 04055977216561