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S7855 CpGenome™ Rat Methylated Genomic DNA Standard

S7855
5 µg per vial  
Purchase on Sigma-Aldrich

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Replacement Information
Description
Catalogue NumberS7855
Brand Family Upstate
Trade Name
  • CpGenome
  • Upstate
DescriptionCpGenome™ Rat Methylated Genomic DNA Standard
OverviewThe CpGenome™ Rat Methylated Genomic DNA Control is an ideal control or standard in DNA methylation studies. This methylated standard is generated using multiple strand displacement amplification of rat genomic DNA using Phi29 DNA polymerase followed by a treatment with a CpG Methylase. This process results in a DNA standard which contains ≥ 95% methylated cytosines across the genome in both heterochromatic and euchromatic regions.
References
Product Information
Presentation5 µg DNA in buffer containing 10mM Tris-HCl pH 8.0, 0.1mM EDTA
Quality LevelMQ100
Applications
ApplicationUseful as a control or standard for variety of epigenomic assays such as methylated or unmethylated DNA enrichment, bisulfite conversion or melt curve analysis to determine the status of global or locus- specific DNA methylation.
Biological Information
Species Reactivity
  • Rat
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceQuantitative Methylation Specific PCR (qMSP):
Bisulfite converted unmethylated and methylated rat genomic DNA are assessed for methylation status at various loci within the genome.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsUpon receipt aliqout and store at -20°C to avoid multiple freeze thaws which can fragment DNA. For best quality, limit freeze and thaw of the same –20°C DNA aliquot to no more than 3 times. For longer term storage, the DNA can be stored at -80°C. With proper storage and protection from excess freeze-thaw cycles, this product is stable for up to two years from the date of receipt.
Packaging Information
Material Size5 µg per vial
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
S7855 04055977118902

Documentation

CpGenome™ Rat Methylated Genomic DNA Standard MSDS

Title

Safety Data Sheet (SDS) 

CpGenome™ Rat Methylated Genomic DNA Standard Certificates of Analysis

TitleLot Number
CpGenomeTM Rat Methylated Genomic DNA Standard 3009707
CpGenomeTM Rat Methylated Genomic DNA Standard 3140150
CpGenomeTM Rat Methylated Genomic DNA Standard 2867042
CpGenomeTM Rat Methylated Genomic DNA Standard - 3172398 3172398
CpGenomeTM Rat Methylated Genomic DNA Standard - 3419561 3419561
CpGenomeTM Rat Methylated Genomic DNA Standard - 3552713 3552713
CpGenomeTM Rat Methylated Genomic DNA Standard - 3817994 3817994
CpGenomeTM Rat Methylated Genomic DNA Standard - DNA Standard DNA STANDARD
CpGenomeTM Rat Methylated Genomic DNA Standard - Q2490102 Q2490102