Centricon® Plus-70 Centrifugal Filter Units
Související zdroje
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Overview
Specifications
Ordering Information
Související produkty a aplikace
Související produkty: Application Facete
| Dialysis and Filtration |
Související produkty: Brand Facete
| Ultracel® |
| Amicon® |
Kategorie
| Life Science Research > Protein Sample Preparation > Protein Concentration & Dialysis > Sample Prep Centrifugal Filter Units |
- 90% typical recovery
- 50X to 200X concentration
- Low hold-up volume
- Polypropylene housing minimizes binding
- Deadstop prevents spinning to dryness
- Ready to use. Nothing to assemble
Centrifugal Filter Units
- Concentrating and desalting chromatography column eluates
- Concentrating monoclonal antibodies
- Concentrating proteins or viruses from culture supernatants
- Clarifying tissue homogenates and cell lysates
- Buffer exchange or diafiltration
Typical Flow Rates
)
Ultracel PL-30 Membrane at 3,500 x g
| Typical Retentate (Concentrate) Recovery | ||
| Membrane NMWL | Protein Solute | Protein Retentate |
| 10,000 | Cytochrome c, 12.4 kDa (0.25 mg/mL) |
94 |
| 30,000 | Bovine Serum Albumin, 67 kDa (1 mg/mL) |
96 |
| 100,000 | IgG, 156 kDa (1 mg/mL) |
91 |
Concentrate samples in the 15 mL-70 mL volume range using Centricon® Plus-70 centrifugal filter units. The large deadstop volume of 350 µL prevents spinning samples to dryness. The Centricon® Plus-70 has two vertical regenerated cellulose membranes for fast, efficient tangential flow. The device is available with four different pore size membranes (3, 10, 30 and 100 kDa NMWL).
Applications
Concentration and Desalting of Chromatography Column Eluates, Concentrating Monoclonal Antibodies, Concentrating Proteins or Viruses From Culture Supernatants, Clarifying Tissue Homogenates and Lysates, Buffer Exchange or Diafiltration
| Volumes | |
| Processing Volume, mL | 15 mL–70 mL |
| Final Concentration Volume, µL | 350 µL |
| Hold-up Volume | 75 µL |
| Centrifugal Force | |
| Materials | |
| Membrane | See ordering information |
| Device | Polypropylene |
| Dimensions | |
| Active Membrane Area, cm2 | 19 cm² |
| Diameter | 6 cm |
| Length | 121 mm |
| Recovery | Typically >90% |
| Sanitization | Rinse with 70% ethanol solution |
| Chemical Compatibility | Use with biological fluids only |