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218813 Caspase-2, Human, Recombinant, E. coli

Caspase-2, Human, Recombinant, E. coli is expressed as a single polypeptide chain that undergoes spontaneous autoprocessing to yield the native active enzyme.

Caspase-2, Human, Recombinant, E. coli MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: ICH-1, Nedd-2

218813
  
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Přehled

Replacement Information
Description
OverviewRecombinant, human caspase-2 expressed in E. coli as a single polypeptide chain. Subsequently undergoes spontaneous auto-processing to yield the native active enzyme. Useful for the study of enzyme regulation, cleavage of target substrates, and inhibitor screening. Cleavage sites are located at Asp316, Asp330, and possibly Asp152. MW: 12000 and 19000.
Catalogue Number218813
Brand Family Calbiochem®
SynonymsICH-1, Nedd-2
References
ReferencesTalanian, R.V., et al. 1997. J. Biol. Chem. 272, 9677.
Wang, L., et al. 1994. Cell 78, 739.
Product Information
Unit of DefinitionOne unit is defined as the amount of enzyme that will cleave 1.0 nmol of Ac-VDVAD-<i>p</i>NA (<a href ="/Products/ProductDisplay.asp→catNO=218820">Cat. No. 218820</a>) per h at 37°C, pH 7.2.
FormLyophilized solid
Quality LevelMQ100
Applications
Biological Information
Purity≥90% by SDS-PAGE
Specific Activity≥1500 units/mg protein
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for up to 6 months at -70°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
218813 0

Documentation

Caspase-2, Human, Recombinant, E. coli MSDS

Title

Safety Data Sheet (SDS) 

Caspase-2, Human, Recombinant, E. coli Certificates of Analysis

TitleLot Number
218813

References

Přehled odkazů
Talanian, R.V., et al. 1997. J. Biol. Chem. 272, 9677.
Wang, L., et al. 1994. Cell 78, 739.

Brochure

Title
Caspases and other Apoptosis Related Tools Brochure
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision04-June-2008 RFH
SynonymsICH-1, Nedd-2
DescriptionRecombinant, human caspase-2 expressed in E. coli as a single polypeptide chain. Subsequently undergoes spontaneous auto-processing to yield the native active enzyme. Useful for the study of enzyme regulation, cleavage of target substrates, and inhibitor screening. Cleavage sites are located at Asp316, Asp330, and possibly Asp152. MW: 12000 and 19000.
FormLyophilized solid
Recommended reaction conditions50 mM HEPES, pH 7.2, 50 mM NaCl, 0.1% CHAPS (Cat. No. 220201), 10 mM EDTA, 5% glycerol, 10 mM DTT, and 200 µM peptide substrate. For analyzing caspase-2 activity, use 1 unit of enzyme per assay.
Purity≥90% by SDS-PAGE
Specific activity≥1500 units/mg protein
Unit definitionOne unit is defined as the amount of enzyme that will cleave 1.0 nmol of Ac-VDVAD-pNA (Cat. No. 218820) per h at 37°C, pH 7.2.
SolubilityPBS (0.1-1 unit/µl)
Storage ≤ -70°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for up to 6 months at -70°C.
Toxicity Standard Handling
ReferencesTalanian, R.V., et al. 1997. J. Biol. Chem. 272, 9677.
Wang, L., et al. 1994. Cell 78, 739.