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218709 Casein Kinase II, α2, Active, Human, Recombinant, E. coli

218709
  
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Description
Overview

This product has been discontinued.



Recombinant, human casein kinase II, α2, (CKII, α2) fused to maltose binding protein (MBP) at the N-terminus and expressed in E. coli. CKII, α2 is a catalytic subunit of the serine/threonine protein kinase CKII. It is suitable for use in reactions where autophosphorylation of the regulatory CKII β-subunit is not desired. Shown to be extremely salt-sensitive with respect to its activity.

Catalogue Number218709
Brand Family Calbiochem®
SynonymsCK2α2, CSNK2A2
Application Data
Increasing concentrations of Casein Kinase II, α2 were assayed according to the protocol outlined above.
References
ReferencesAntonelli, M., et al. 1997. Eur. J. Biochem. 241, 272.
Xu, X., et al. 1997. Genomics 48, 79.
Boldyreff, B., et al. 1994. Cell. Mol. Biol. Res. 40, 391.
Product Information
Unit of DefinitionOne unit is defined as the amount of enzyme required to transfer 1 pmol phosphate to RRRADDSDDDDD peptide substrate per min at 30°C.
FormLiquid
FormulationIn 50 mM Tris-HCl, 500 mM NaCl, 10 mM DTT, pH 8.5.
Quality LevelMQ100
Biological Information
Purity≥ 90% by SDS PAGE
Specific Activity≥841.819 Units/mg protein
Concentration Label Please refer to vial label for lot-specific concentration
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Global Trade Item Number
Katalogové číslo GTIN
218709 0