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SCT205 BioTracker™ EP-HMRG Green Protease Live Cell Probe

Live cell imaging probe that reacts with the proteases that digest the peptide sequence of EP (glutamic acid, proline) to fluoresce green by fluorescence microscopy or flow cytometry.

SCT205
5 ea x 30 nmol  
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Replacement Information
Description
Catalogue NumberSCT205
DescriptionBioTracker™ EP-HMRG Green Protease Live Cell Probe
OverviewBioTracker™ EP-HMRG is nearly nonfluorescent at pH 7 or higher. It fluoresces by the reaction with proteases that specifically digest the EP (glutamic acid, proline) peptide sequence. A green fluorescent product is membrane permeable and localizes to the lysosome.

SCT205 exhibits no or very low fluorescence at pH >= 7. It fluoresces by the reaction with proteases that digest the peptide sequces of EP (glutamic acid, proline).

SPECTRAL PROPERTIES:
Excitation: 500 nm
Emission: 524 nm
For fluorescence microscopy, blue excitation filter cubes for FITC or GFP are suitable.

REFERENCE

Onoyama H, Kamiya M, Kuriki Y, Komatsu T, Abe H, Tsuji Y, Yagi K, Yamagata Y, Aikou S, Nishida M, et al. 2016. Rapid and sensitive detection of early esophageal squamous cell carcinoma with fluorescence probe targeting dipeptidylpeptidase IV. Scientific Reports. 6(1):26399.
Alternate Names
  • EP-HMRG probe
  • EP-HMRG live cell probe
  • protease live cell probe
  • EP protease probe
Background InformationEarly detection of esophageal squamous cell carcinoma (ESCC) is an important for a favorable prognosis, but is difficult to achieve by conventional endoscopy. Since fluorescence-based visualization is considered a promising approach, development of an activatable fluorescence probe to visualize ESCCs became an urgent goal. Based on the fact that various aminopeptidase activities are elevated in cancer, specimens freshly resected from patients were screened with aminopeptidase-activatable fluorescence probes. The results indicated that dipeptidylpeptidase IV (DPP-IV) is specifically activated in ESCCs and would be an appropriate molecular target for detection of esophageal cancer. DPP-IV-targeted activatable fluorescence probes may prove useful tools for detection of cancers characterized by EP (glutamic acid-proline) sequences such as that found in DPP-IV (dipeptidyl peptidase.)
References
Product Information
Detection methodFlow cytometry Fluorescence
FormatLyophilized solid
PresentationLyophilized. Orange solid.
Quality LevelMQ100
Applications
ApplicationLive cell imaging probe that reacts with the proteases that digest the peptide sequence of EP (glutamic acid, proline) to fluoresce green by fluorescence microscopy or flow cytometry.
Key Applications
  • Protease assays
  • Cell Based Assays
  • Flow Cytometry
  • Fluorescence
Application Notes
To use SCT205 EP-HMRG protease live cell probe, warm the vial to room temperature and add 30 μL of DMSO or PBS to one vial to make 1 mM solution.
Dilute the stock solution with a neutral buffer or culture medium to 1-2 uM and use the diluted solution immediately.

NOTE: Under acidic conditions, BioTracker™ EP-HMRG Green Protease Live Cell Probe emits fluorescence
by a different mechanism from protease hydrolysis. It therefore must be diluted with a neutral or weakly basic solution (pH>7).

Storage of the reconstituted probe solution is not recommended. If it becomes necessary to store temporarily, keep aqueous solution on ice, or freeze DMSO solution and use as quickly as possible.
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore BioTracker™ EP-HMRG Live Cell Probe at -20°C, desiccated and protected from light. Centrifuge vial briefly to collect contents at bottom of vial before opening.
NOTE: Storage of reconstituted probe is not recommended. For best results, discard unused probe solution and make fresh for each use.
Packaging Information
Material Size5 ea x 30 nmol
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
SCT205 04065270309136