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07-2115 Anti-phospho-RSK4 Antibody (Ser372)

This phospho-RSK4 antibody is validated for use in WB for the detection of the phospho-RSK4 protein.

Anti-phospho-RSK4 Antibody (Ser372) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: MAP Kinases Gene Symbol: PP90RSK4, RSK-4, RSK4, pp90RSK4 UniProt Number: Q9UK32
07-2115
100 µg  
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Tabulka spec. kláve

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, RWBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue Number07-2115
DescriptionAnti-phospho-RSK4 Antibody (Ser372)
Alternate Names
  • 90 kDa ribosomal protein S6 kinase 6
  • Ribosomal S6 kinase 4
  • S6K-alpha 6
  • p90-RSK 6
  • ribosomal protein S6 kinase polypeptide 6
  • ribosomal protein S6 kinase, 90kD, polypeptide 6
  • ribosomal protein S6 kinase, 90kDa, polypeptide 6
Background InformationRSK1, RSK2, RSK3, and RSK4 (ribosomal protein S6 kinase, 90 kDa, polypeptide 1,2,3; S6K-alpha 1,2,3) are Ser/Thr kinases which are activated by MAP Kinases. Ribosomal S6-kinase 4 (RSK4) is thought to be mapped in the critical region that is commonly deleted in X-linked deafness type 3, mental retardation, and choroideremia. It may be involved in normal neuronal development. RSK4 is also thought to play a role in non-growth factor signaling and may also be important in tumor suppression.
References
Product Information
FormatAffinity Purified
Control
  • EGF treated and untreated PC-12 cell lysates
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationThis phospho-RSK4 antibody is validated for use in WB for the detection of the phospho-RSK4 protein.
Key Applications
  • Western Blotting
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to human RSK4 phosphorylated at Ser372.
EpitopePhosphorylated Ser372
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityThis antibody recognizes RSK4 phosphorylated at Ser372.
Species Reactivity
  • Human
  • Mouse
  • Rat
Species Reactivity NoteDemonstrated to react with human. Predicted to react with mouse and rat based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThis gene encodes a member of ribosomal S6 kinase family, serine-threonine protein kinases which are regulated by growth factors. The encoded protein may be distinct from other members of this family, however, as studies suggest it is not growth factor dependent and may not participate in the same signaling pathways.
Gene Symbol
  • PP90RSK4
  • RSK-4
  • RSK4
  • pp90RSK4
Modifications
  • Phosphorylation
Purification MethodAffinity Purfied
UniProt Number
UniProt SummaryFUNCTION: Serine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB.

CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.

COFACTOR: Magnesium (By similarity).

ENZYME REGULATION: Activated by multiple phosphorylations on threonine and serine residues (By similarity).

SUBUNIT STRUCTURE: Forms a complex with either ERK1 or ERK2 in quiescent cells. Transiently dissociates following mitogenic stimulation (By similarity).

PTM: Autophosphorylated on Ser-389, as part of the activation process (By similarity).

SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.

Contains 1 AGC-kinase C-terminal domain.

Contains 2 protein kinase domains.
Molecular Weight ~80 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in EGF treated and untreated PC-12 cell lysates.

Western Blot Analysis: 2 µg/mL of this antibody detected RSK4 on 10 µg of EGF treated and untreated PC-12 cell lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
07-2115 04053252292125