Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

MABE229 Anti-phospho-Cdk1 (Thr14, Tyr15)Antibody, clone CP3.2

Anti-phospho-Cdk1 (Thr14, Tyr15) Antibody, clone CP3.2 is a mouse monoclonal antibody for detection of phospho-Cdk1 (Thr14, Tyr15) also known as Cyclin-dependent kinase 1, or Cell division control protein 2 homolog & has been validated in WB, ICC.

Anti-phospho-Cdk1 (Thr14, Tyr15)Antibody, clone CP3.2 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Cell Cycle, DNA Replication & Repair Gene Symbol: CDK1, CDC2, CDC28A, CDKN1, P34CDC2 UniProt Number: P06493
MABE229
100 µL  
Purchase on Sigma-Aldrich

Speciální nabídky

Přehled

Replacement Information

Speciální nabídky

100% Performance Guaranteed

Tabulka spec. kláve

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, ICCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABE229
DescriptionAnti-phospho-Cdk1 (Thr14, Tyr15)Antibody, clone CP3.2
Alternate Names
  • Cyclin-dependent kinase 1
  • CDK1
  • Cell division control protein 2 homolog
  • Cell division protein kinase 1
  • p34 protein kinase
Background InformationCyclin dependent kinase 1 (Cdk1) regulates cell cycle progression. It is active as a heterodimeric complex with cyclins. Cdk1 associates with cyclin A at the end of S phase and targets proteins such as p53, BARD1, and BRCA2 involved in cell cycle progression, and others involved in DNA replication. At the end of the G2 phase, Cdk1 associates with cyclin B1 and B2 to phosphorylate multiple protein targets required for transition from G2 to M phase, and progression through M phase. Cdk1 itself undergoes phosphorylation at Thr14 and Tyr15 by PKMYT1, which prevents nuclear translocation of Cdk1. Cdk1 is also phosphorylated at Tyr15 by WEE1/2 which inhibits both Cdk1 activity and transition from G2 to M phase. Conversely, dephosphorylation of Thr14 and Tyr15 by CDC25A/B activates Cdk1 and triggers mitosis. Cdk1 may also regulate epigenetic gene silencing through phosphorylation of EZH2 and maintenance of histone H3 lysine 27 trimethylation, which has been implicated in cancer.
References
Product Information
FormatPurified
Control
  • UV treated and untreated HeLa cell lysates
PresentationPurified mouse monoclonal IgG1κ supernatant in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-phospho-Cdk1 (Thr14, Tyr15) Antibody, clone CP3.2 is a mouse monoclonal antibody for detection of phospho-Cdk1 (Thr14, Tyr15) also known as Cyclin-dependent kinase 1, or Cell division control protein 2 homolog & has been validated in WB, ICC.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Biological Information
ImmunogenLinear peptide corresponding to human Cdk1 phosphorylated at Thr14 and phosphorylated at Tyr15.
EpitopePhosphorylated Thr14 and phosphorylated Tyr15
CloneCP3.2
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityThis antibody recognizes Cdk1 phosphorylated at Thr14 and phosphorylated at Tyr15.
IsotypeIgG1κ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • CDK1
  • CDC2
  • CDC28A
  • CDKN1
  • P34CDC2
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~34 kDa observed. An uncharacterized band may be observed at ~62 kDa in some cell lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in UV treated and untreated HeLa cell lysates.

Western Blot Analysis: A 1:1,000 dilution from a representative lot detected phospho-Cdk1 (Thr14, Tyr15) in UV treated HeLa cell lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
MABE229 04053252694752

Documentation

Anti-phospho-Cdk1 (Thr14, Tyr15)Antibody, clone CP3.2 MSDS

Title

Safety Data Sheet (SDS) 

Anti-phospho-Cdk1 (Thr14, Tyr15)Antibody, clone CP3.2 Certificates of Analysis

TitleLot Number
Anti-phospho-Cdk1 (Thr14, Tyr15), clone CP3.2 - 2435676 2435676
Anti-phospho-Cdk1 (Thr14, Tyr15), clone CP3.2 - 2494271 2494271
Anti-phospho-Cdk1 (Thr14, Tyr15), clone CP3.2 - 3252817 3252817
Anti-phospho-Cdk1 (Thr14, Tyr15), clone CP3.2 - 3514952 3514952
Anti-phospho-Cdk1 (Thr14, Tyr15), clone CP3.2 - 3915638 3915638
Anti-phospho-Cdk1 (Thr14, Tyr15), clone CP3.2 - 4113061 4113061
Anti-phospho-Cdk1 (Thr14, Tyr15), clone CP3.2 - Q2040038 Q2040038
Anti-phospho-Cdk1 (Thr14, Tyr15), clone CP3.2 -2523880 2523880
Anti-phospho-Cdk1 (Thr14, Tyr15), clone CP3.2 -2763835 2763835
Anti-phospho-Cdk1 (Thr14, Tyr15), clone CP3.2 Monoclonal Antibody 2924635
Show more