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PC373 Anti-p40 (5-17) Rabbit pAb

This Anti-p40 (5-17) Rabbit pAb is validated for use in Immunoblotting, Paraffin Sections for the detection of p40 (5-17).

Anti-p40 (5-17) Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
PC373
  
Purchase on Sigma-Aldrich

Přehled

Replacement Information

Tabulka spec. kláve

Species ReactivityHostAntibody Type
HRbPolyclonal Antibody
Description
OverviewRecognizes the ~40 kDa p40 protein in SKOV3 cells and breast carcinoma tissue.
Catalogue NumberPC373
Brand Family Calbiochem®
Application Data
Detection of human p40 by staining paraffin sections. Sample: Human breast carcinoma. Primary antibody: Anti-p40 (5-17) Rabbit pAb (Cat. No. PC373) (1:3000). Detection: DAB with methyl green counterstain.
References
ReferencesHibi, K., et al. 2000. Proc. Natl. Acad. Sci. USA 97, 5462.
Yamaguchi, K., et al. 2000. Int. J. Cancer 86, 684.
Hagiwara, K.,et al. 1999. Cancer Res. 59, 4165.
Kaelin W. G. Jr. 1999. J. Natl. Cancer Inst. 91, 594.
Osada, M., et al. 1998. Nat. Med. 7, 839.
Senoo, M., et al. 1998. Biochem. Biophys. Res. Commun. 248, 603.
Trink, B., et al. 1998. Nat. Med. 4, 747.
Yang, A., et al. 1998. Mol. Cell 2, 305.
Product Information
FormLiquid
FormulationUndiluted serum.
Positive controlSKOV3 cells or breast carcinoma tissue
PreservativeNone
Quality LevelMQ100
Applications
Key Applications Immunoblotting (Western Blotting)
Not Immunofluorescence
Paraffin Sections
Application NotesImmunoblotting (1:500, chemiluminescence)
Paraffin Sections (1:3000, heat pre-treatment required)
Application CommentsAntibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena synthetic peptide corresponding to amino acids 5-17 of human p40
ImmunogenHuman
HostRabbit
IsotypeIgG
Species Reactivity
  • Human
Antibody TypePolyclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
PC373 0

Documentation

Anti-p40 (5-17) Rabbit pAb MSDS

Title

Safety Data Sheet (SDS) 

Anti-p40 (5-17) Rabbit pAb Certificates of Analysis

TitleLot Number
PC373

References

Přehled odkazů
Hibi, K., et al. 2000. Proc. Natl. Acad. Sci. USA 97, 5462.
Yamaguchi, K., et al. 2000. Int. J. Cancer 86, 684.
Hagiwara, K.,et al. 1999. Cancer Res. 59, 4165.
Kaelin W. G. Jr. 1999. J. Natl. Cancer Inst. 91, 594.
Osada, M., et al. 1998. Nat. Med. 7, 839.
Senoo, M., et al. 1998. Biochem. Biophys. Res. Commun. 248, 603.
Trink, B., et al. 1998. Nat. Med. 4, 747.
Yang, A., et al. 1998. Mol. Cell 2, 305.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision09-October-2007 RFH
ApplicationImmunoblotting (1:500, chemiluminescence)
Paraffin Sections (1:3000, heat pre-treatment required)
Application Data
Detection of human p40 by staining paraffin sections. Sample: Human breast carcinoma. Primary antibody: Anti-p40 (5-17) Rabbit pAb (Cat. No. PC373) (1:3000). Detection: DAB with methyl green counterstain.
DescriptionRabbit polyclonal antibody supplied as undiluted serum. Recognizes the ~40 kDa p40 protein.
BackgroundAmong the more recently identified p53 homologues are p73 and p40/p63/p73L/p51/CUSP/KET. Existing as two splice variants with molecular weights of ~51 kDa and ~72 kDa, the p40 gene product is essential for normal epithelial development. The gene maps to the distal arm of chromosome 3q and is mutated in a diverse number of human cancers. The p40 gene is expressed in skeletal muscle, placenta, mammary gland, prostate, trachea, thymus, salivary gland, uterus, heart and lung. The p40 protein has the ability to transactivate many p53 reporter genes and also has the ability to induce apoptosis.
HostRabbit
Immunogen speciesHuman
Immunogena synthetic peptide corresponding to amino acids 5-17 of human p40
IsotypeIgG
Specieshuman, not mouse
Positive controlSKOV3 cells or breast carcinoma tissue
FormLiquid
FormulationUndiluted serum.
PreservativeNone
CommentsAntibody should be titrated for optimal results in individual systems.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesHibi, K., et al. 2000. Proc. Natl. Acad. Sci. USA 97, 5462.
Yamaguchi, K., et al. 2000. Int. J. Cancer 86, 684.
Hagiwara, K.,et al. 1999. Cancer Res. 59, 4165.
Kaelin W. G. Jr. 1999. J. Natl. Cancer Inst. 91, 594.
Osada, M., et al. 1998. Nat. Med. 7, 839.
Senoo, M., et al. 1998. Biochem. Biophys. Res. Commun. 248, 603.
Trink, B., et al. 1998. Nat. Med. 4, 747.
Yang, A., et al. 1998. Mol. Cell 2, 305.