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ABE1363 Anti-acetyl Histone H2A.Z (Lys 5, 7, 11) Antibody

This Anti-acetyl Histone H2A.Z (Lys 5, 7, 11) Antibody is validated for use in Western Blotting and Immunocytochemistry and Chromatin Immunoprecipitation (ChIP) and ChIP-seq and Dot Blot and ELISA for

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Anti-acetyl Histone H2A.Z (Lys 5, 7, 11) Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Nuclear Receptors Gene Symbol: H2AFZ, H2AZ UniProt Number: P0C0S5
ABE1363
50 µg  
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Speciální nabídky

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Tabulka spec. kláve

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MWB, ICC, ChIP, ChIP-seq, DB, ELISARbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABE1363
DescriptionAnti-acetyl Histone H2A.Z (Lys 5, 7, 11) Antibody
Alternate Names
  • Histone H2A.Z
  • H2A/z
Background InformationH2A.Z, also known as H2AFZ, is a variant of histone H2A that is highly conserved from yeast to humans. Like other histone variants, H2A.Z is encoded by a unique gene, resulting in a slightly divergent type of H2A with specialized functions. H2A.Z is involved in chromatin compaction by stabilizing the histone octamer within the nucleosome. It promotes gene expression through the creation of an open domain of chromatin that is more easily transcribed. H2A.Z is nonrandomly distributed throughout the genome, targeted primarily to pericentric heterochromatin, but excluded from the inactive X chromosome and the nucleolus. H2A.Z is associated with both euchromatin and facultative heterochromatin. DNA methylation can influence chromatin structure and effect gene silencing by excluding H2A.Z, and likewise, H2A.Z protects genes from DNA methylation. Thus, H2A.Z plays a role in regulating heterochromatin silencing and is involved in transcriptional control.
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal in buffer containing PBS with 0.05% sodium azide and 0.05% ProClin300.
Quality LevelMQ100
Applications
ApplicationThis Anti-acetyl Histone H2A.Z (Lys 5, 7, 11) Antibody is validated for use in Western Blotting and Immunocytochemistry and Chromatin Immunoprecipitation (ChIP) and ChIP-seq and Dot Blot and ELISA for the detection of acetyl Histone H2A.Z (Lys 5, 7, 11).
Key Applications
  • Western Blotting
  • Immunocytochemistry
  • Chromatin Immunoprecipitation (ChIP)
  • ChIP-seq
  • Dot Blot
  • ELISA
Application NotesChromatin Immunoprecipitation Analysis: A representative lot immunoprecipitated acetyl Histone H2A.Z (Lys 5, 7, 11) using chromatin from HeLa cells.
Dot Blot Analysis: 0.05 ug/mL from a representative lot detected acetyl Histone H2A.Z (Lys 5, 7, 11) in H2A.Z peptides.
Chromatin Immunoprecipitation (Seq.) Analysis: A representative lot immunoprecipitated acetyl Histone H2A.Z (Lys 5, 7, 11) using chromatin from K562 cells.
Immunocytochemistry Analysis: A representative lot detected acetyl Histone H2A.Z (Lys 5, 7, 11) in HeLa cells.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to human acetyl Histone H2A.Z (Lys 5, 7, 11) near the N-terminus.
EpitopeNear N-terminus
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityHuman, mouse, wide range expected.
Species Reactivity
  • Human
  • Mouse
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • H2AFZ
  • H2AZ
Modifications
  • Acetylation
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~16 kDa observed
Product Usage Statements
Quality AssuranceWestern Blotting Analysis: 0.5 µg/mL from a representative lot detected acetyl Histone H2A.Z (Lys 5, 7, 11) in 10 µg of sodium butyrate treated HeLa acid extract.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size50 µg
Global Trade Item Number
Katalogové číslo GTIN
ABE1363 04055977073256