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ABE1976 Anti-XPC Antibody

This Anti-XPC Antibody is validated for use in Western Blotting for the detection of XPC.

Anti-XPC Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Cell Cycle, DNA Replication & Repair Gene Symbol: RAD4, XP3, XPC, XPCC UniProt Number: Q01831
ABE1976
100 µL  
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Speciální nabídky

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Tabulka spec. kláve

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MWBRbSerumPolyclonal Antibody
Description
Catalogue NumberABE1976
DescriptionAnti-XPC Antibody
Alternate Names
  • DNA repair protein complementing XP-C cells
  • xeroderma pigmentosum group C-complementing protein
  • p125
Background InformationDNA repair protein complementing XP-C cells (UniProt Q01831; also known as xeroderma pigmentosum group C-complementing protein, p125) is encoded by the XPC (also known as RAD4, XP3, XPCC) gene (Gene ID 7508) in human. Nucleotide excision repair (NER) removes damage to DNA caused by UV and mutagens. The NER process involves damage recognition and formation of an open DNA structure around the lesion, followed by dual incision, excision of an oligonucleotide of 24 to 32 residues, and gap-filling DNA synthesis to restore the DNA molecule. In human, the minimal set of components involved in NER includes replication protein A (RPA), XPA, XPC-RAD23B, XPG, ERCC1-XPF, TFIIH, replication factor C (RFC), PCNA, DNA polymerase δ or ɛ, and DNA ligase I. These factors can be divided into two groups, the six core factors necessary for damage recognition and dual incision (RPA, XPA, XPC-RAD23B complex, XPG, ERCC1-XPF, and TFIIH) and those for repair DNA synthesis in the gap-filling step. XPC is specifically involved in global genome NER (GG-NER) and serves as a damage recognition protein in complex with RAD23B. XPC is the rate-limiting factor in the GG-NER process, and XPC-dependent distortion recognition plays an essential role in initiating the recruitment of core NER proteins to the lesion.
References
Product Information
FormatSerum
PresentationRabbit polyclonal serum with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-XPC Antibody is validated for use in Western Blotting for the detection of XPC.
Key Applications
  • Western Blotting
Application NotesWestern Blotting Analysis: A representative lot detected XPC in CBP-HMGB1 affinity pull-downs from XP12RO human xeroderma pigmentosum cell lysates (Lange, S.S., et al. (2009). DNA Repair (Amst). 8(7):865-872).
Western Blotting Analysis: A representative lot detected XPC as one of the nucleotide excision repair (NER) proteins present in the immunoprecipitated TFIIH complex from HeLa cell lysates (Araújo, S.J., et al. (2001). Mol. Cell Biol. 21(7):2281-2291).
Biological Information
ImmunogenRecombinant fragment corresponding to human XPC amino acids 96-299.
EpitopeAmino acids 96-299.
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityExpected to react with all three spliced isoforms reported by UniProt (Q01831). This polyclonal antibody is known as RW028 in publications.
Species Reactivity
  • Human
  • Mouse
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • RAD4
  • XP3
  • XPC
  • XPCC
Purification MethodUnpurified
UniProt Number
Molecular Weight~125 kDa observed. 106.0 kDa (isoform 1) 101.8 kDa (isoform 2) and 15.71 kDa (isoform 3) calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in LNCaP nuclear extract.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected XPC in 10 µg of LNCaP nuclear extract.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
ABE1976 04055977314908

Documentation

Anti-XPC Antibody Certificates of Analysis

TitleLot Number
Anti-XPC -Q2642754 Q2642754