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ABE555 Anti-WTAP Antibody

Anti-WTAP, Cat. No. ABE555, is a highly specific rabbit polyclonal antibody that targets WTAP and has been tested in Western Blotting.

Anti-WTAP Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: WTAP, KIAA0105 UniProt Number: Q15007
ABE555
100 µL  
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Tabulka spec. kláve

Species ReactivityKey ApplicationsHostFormatAntibody Type
M, H, RWBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABE555
DescriptionAnti-WTAP Antibody
Alternate Names
  • Pre-mRNA-splicing regulator WTAP
  • Female-lethal(2)D homolog
  • hFL(2)D
  • Wilms tumor 1-associating protein
  • WT1-associated protein
Background InformationPre-mRNA-splicing regulator WTAP (UniProt Q15007; also known as Female-lethal (2)D homolog, hFL(2)D, Wilms tumor 1-associating protein, WT1-associated protein) is encoded by the WTAP (also known as KIAA0105) gene (Gene ID 9589) in human. WTAP is an ubiquitously expressed nuclear protein that is localized throughout the nucleoplasm and in speckles and partially co-localizes with splicing factors. A mouse homolog has also been reported that shares 96% sequence identity. WTAP regulates G2/M cell-cycle transition by binding to the 3' UTR of CCNA2, which enhances its stability. WTAP knockdown is shown to induce G2 accumulation, which is partially rescued by adenoviral overexpression of cyclin A2. WTAP-null mice exhibit proliferative failure with early embryonic death. WTAP is also reported to be an oncogenic protein in acute myeloid leukemia (AML) and its increased expression has been linked to chemoresistance in AML. Ref: Horiuchi, K., et al. (2006), Proc. Natl. Acad. Sci. USA 103, 17278-17283. Bansal, H., et al. (2014). Leukemia 28, 1171-1174.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-WTAP, Cat. No. ABE555, is a highly specific rabbit polyclonal antibody that targets WTAP and has been tested in Western Blotting.
Key Applications
  • Western Blotting
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to an internal sequence within the N-terminal half of human WTAP.
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis polyclonal antibody targets an internal sequence within the N-terminal half of human WTAP.
Species Reactivity
  • Mouse
  • Human
  • Rat
Species Reactivity NoteHuman, Mouse, Rat.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • WTAP
  • KIAA0105
Purification MethodAffinity purified.
UniProt Number
Molecular Weight~52 observed. 44.24/17.80 kDa (isoform 1/2 calculated). Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in A431.

Western Blotting Analysis: A 1:250 dilution of this antibody detected WTAP in 10 µg of A431 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
ABE555 04054839092046