Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

MABF2337-100UG Anti-VSV-Glycoprotein Antibody, clone 8G5F11

MABF2337-100UG
100 μg  
Purchase on Sigma-Aldrich

Přehled

Replacement Information
Description
Catalogue NumberMABF2337-100UG
DescriptionAnti-VSV-Glycoprotein Antibody, clone 8G5F11
Alternate Names
  • Glycoprotein Vesicular stomatitis Indiana virus
  • Vesicular stomatitis virus glycoprotein
  • VSIV Glycoprotein
Background InformationGlycoprotein from Vesicular stomatitis virus (UniProt: P03522; also known as VSV) is encoded by the G gene (Gene ID: 1489834) in this virus. VSV belongs to the Rhabdoviridae family and two distinct serotypes of this virus have been described the New Jersey and Indiana type. It is an enveloped, negative-sense RNA virus that mainly infects a variety domestic animals and causes vesiculation, ulceration, and erosion of the oral and nasal mucosa and epithelial surface of the tongue. The genomic structure of this virus is a single strand of negative-sense RNA that is composed of five genes known as N, P, M, G, and L that represent the nucleocapsid protein, phosphoprotein, matrix protein, glycoprotein, and the large protein, which is a component of the viral RNA polymerase. The virus glycoprotein is a single pass type 1 membrane protein that attaches virus to host LDL receptors and induces clathrin-dependent endocytosis of the virion. In the endosome, the acidic pH induces conformational changes in the glycoprotein trimer that triggers fusion between virus and endosomal membrane. Because the VSV-Glycoprotein can bind to numerous cell types, it is used for the transduction of genetic material into cells as a vaccine vector and as an antitumor agent. The glycoprotein consists of a virion surface domain (aa 17-467), a helical domain (aa 468-488), and intra-virion domain (aa 489-511). Clone 8G5G11 is shown to cross-react with and cross-neutralize a variety of vesiculovirus members. The DKD residues (aa 257-259) of glycoprotein are shown to dictate binding of this antibody. Clone 8G5F11 is reported to block infection after endocytosis and before genome reverse transcription. (Ref.: Munis, AM., et al. (2018). J. Virol. 92(23); e00900-18).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2a in PBS without azide.
Applications
ApplicationAnti-VSV-Glycoprotein, clone 8G5F11, Cat. No. MABF2337, is a mouse monoclonal antibody that detects VSV-Glycoprotein and is tested in Flow Cytometry, Inhibition Assay, Neutralization, Radioimmunoassay, and Western Blotting.
Key Applications
  • Flow Cytometry
  • Inhibition
  • Neutralization Assay
  • Radioimmunoassay
  • Western Blotting
Application NotesFlow Cytometry Analysis: A representative lot detected VSV-Glycoprotein in Flow Cytometry applications (Cole, N.B., et. al. (1998). J Cell Biol. 140(1):1-15. 5; Munis, A.M., et. al. (2018). J Virol. 92(23); e00900-18).

Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected VSV-Glycoprotein in BHK cell lysates infected with VSV-Orsay (Courtesy of Shelby Puckett (Lyles Lab), Wake Forest School of Medicine, North Carolina USA).

Radioimmunoassay Analysis: A representative lot detected VSV-Glycoprotein in Radioimmunoassay applications (Lefrancois, L., et. al. (1982). Virology. 121(1):157-67).

Inhibition Analysis: A representative lot inhibited VSV, Indiana Strain with an IC50 of 11.5 ng/mL. (Munis, A.M., et. al. (2018). J Virol. 92(23); e00900-18).

Neutralizing Analysis: A representative lot cross-neutralizes Vesicular stomatitis virus Indiana strain G by inhibiting it. (Munis, A.M., et. al. (2018). J Virol. 92(23); e00900-18; Rogalin, H.B., et. al. (2016). J Virol. 90(22); 10321-10328).

Western Blotting Analysis: A representative lot detected VSV-Glycoprotein in Western Blotting applications (Lefrancois, L., et. al. (1982). Virology. 121(1):157-67).
Biological Information
ImmunogenVesicular stomatitis virus, Indiana serotype
Epitopeextracellular domain
Clone8G5F11
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 8G5F11 is a mouse monoclonal antibody that detects Glycoprotein of Vesicular stomatitis and also binds to and neutralizes G proteins from three strains of VSV, as well as Cocal and Maraba viruses in infected cells.
IsotypeIgG2aκ
Species Reactivity
  • Virus
Species Reactivity NoteVirus (Vesicular stomatitis).
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • G
Purification MethodProtein G purified
UniProt Number
Molecular Weight57.48 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceIsotype testing: Identity Confirmation by Isotyping Test.

Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG2a .
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
MABF2337-100UG 04061841440826

Documentation

Anti-VSV-Glycoprotein Antibody, clone 8G5F11 MSDS

Title

Safety Data Sheet (SDS) 

Anti-VSV-Glycoprotein Antibody, clone 8G5F11 Certificates of Analysis

TitleLot Number
Anti-VSV-Glycoprotein, clone 8G5F11 - 3532371 3532371
Anti-VSV-Glycoprotein, clone 8G5F11 - 3536128 3536128
Anti-VSV-Glycoprotein, clone 8G5F11 - 3540396 3540396
Anti-VSV-Glycoprotein, clone 8G5F11 - 3552716 3552716
Anti-VSV-Glycoprotein, clone 8G5F11 - 3560030 3560030
Anti-VSV-Glycoprotein, clone 8G5F11 - 3563206 3563206
Anti-VSV-Glycoprotein, clone 8G5F11 - 3574420 3574420
Anti-VSV-Glycoprotein, clone 8G5F11 - 3584377 3584377
Anti-VSV-Glycoprotein, clone 8G5F11 - 3666028 3666028
Anti-VSV-Glycoprotein, clone 8G5F11 - 3669260 3669260

References

Reference overviewPub Med ID
Characterization of Antibody Interactions with the G Protein of Vesicular Stomatitis Virus Indiana Strain and Other Vesiculovirus G Proteins.
Munis, AM; Tijani, M; Hassall, M; Mattiuzzo, G; Collins, MK; Takeuchi, Y
J Virol  92  N/A  2018

Zobrazit abstrakt
30232190 30232190
Characterization of Vesicular Stomatitis Virus Pseudotypes Bearing Essential Entry Glycoproteins gB, gD, gH, and gL of Herpes Simplex Virus 1.
Rogalin, HB; Heldwein, EE
J Virol  90  10321-10328  2015

Zobrazit abstrakt
27605677 27605677
Retrograde transport of Golgi-localized proteins to the ER.
Cole, NB; Ellenberg, J; Song, J; DiEuliis, D; Lippincott-Schwartz, J
J Cell Biol  140  1-15  1998

Zobrazit abstrakt
9425149 9425149
The interaction of antibody with the major surface glycoprotein of vesicular stomatitis virus. I. Analysis of neutralizing epitopes with monoclonal antibodies.
Lefrancois, L; Lyles, DS
Virology  121  157-67  1981

Zobrazit abstrakt
18638751 18638751