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05-829-AF647 Anti-α-Tubulin Antibody, clone DM1A, Alexa Fluor® 647 conjugate

05-829-AF647
100 µL  
Purchase on Sigma-Aldrich

Speciální nabídky

Přehled

Replacement Information

Speciální nabídky

Tabulka spec. kláve

Species ReactivityKey ApplicationsHostFormatAntibody Type
M, H, Gp, Av, B, Porcine, Gr, RICCMAlexaFluor®647Monoclonal Antibody
Description
Catalogue Number05-829-AF647
DescriptionAnti-α-Tubulin Antibody, clone DM1A, Alexa Fluor® 647 conjugate
Alternate Names
  • Tubulin alpha-1 chain
  • α-Tubulin
Background InformationTubulin alpha-1 chain (UniProt P02552) is encoded by the TUBA1C (also known as TUBA1) gene (Gene ID 429035) in Gallus gallus (Chicken) species. Microtubules are cytoskeletal filaments composed of alpha-/beta-tubulin heterodimers that self-assemble head-to-tail to form protofilaments and laterally to form a hollow tube. Various post-translational modifications, including acetylation, polyglutamylation, polyglycylation, detyrosination, phosphorylation, and palmitoylation, can regulate the polymerization properties of tubulins and/or their interactions with microtubule associated proteins (MAPs) and motor proteins.
References
Product Information
FormatAlexaFluor®647
PresentationPurified mouse monoclonal IgG1 antibody conjugate in PBS with 15 mg/mL BSA and 0.1 % sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-α-Tubulin Antibody, clone DM1A, Alexa Fluor® 647 conjugate is an antibody against α-Tubulin for use in Immunocytochemistry.
Key Applications
  • Immunocytochemistry
Application NotesImmunocytochemistry Analysis: A 1:100 dilution from a representative lot detected α-Tubulin in NIH/3T3 cells.
The unconjugated antibody (Cat. No. 05-829) is shown to be suitable also for immunocytochemistry, immunofluorescence, and Western blotting applications.
Biological Information
ImmunogenPurified chicken brain α-Tubulin. The epitope recognized by this antibody has been mapped to the C-terminal 25 amino acids (AALEKDYEEVGVDSVEGEGEEEGEE).
EpitopeC-terminal 25 amino acids.
CloneDM1A
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone DM1A recognizes α-Tubulin, MW 50-60 kDa. The epitope recognized by clone DM1A has been mapped to the C-terminal 25 amino acids.
IsotypeIgG1
Species Reactivity
  • Mouse
  • Human
  • Guinea Pig
  • Avian
  • Bovine
  • Porcine
  • Gerbil
  • Rat
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • TUBA1C
  • TUBA1
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~50 kDa observed.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunocytochemistry in A431 cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected α-Tubulin in A431 cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
05-829-AF647 04055977330571

Documentation

Anti-α-Tubulin Antibody, clone DM1A, Alexa Fluor® 647 conjugate MSDS

Title

Safety Data Sheet (SDS) 

Anti-α-Tubulin Antibody, clone DM1A, Alexa Fluor® 647 conjugate Certificates of Analysis

TitleLot Number
Anti-α-Tubulin, clone DM1A, -Q2662538 Q2662538
Anti-α-Tubulin, clone DM1A, Alexa Fluor® 647 conjugate - 3217766 3217766
Anti-α-Tubulin, clone DM1A, Alexa Fluor® 647 conjugate - 3539741 3539741
Anti-α-Tubulin, clone DM1A, Alexa Fluor® 647 conjugate - 3810421 3810421
Anti-α-Tubulin, clone DM1A, Alexa Fluor® 647 conjugate - 3852427 3852427
Anti-α-Tubulin, clone DM1A, Alexa Fluor® 647 conjugate - 4093602 4093602
Anti-α-Tubulin, clone DM1A, Alexa Fluor® 647 conjugate - 4181448 4181448
Anti-α-Tubulin, clone DM1A, Alexa Fluor® 647 conjugate Monoclonal Antibody 3137642
Anti-α-Tubulin, clone DM1A, Alexa Fluor® 647 conjugate Monoclonal Antibody 2949133
Anti-α-Tubulin, clone DM1A, Alexa Fluor® 647 conjugate Monoclonal Antibody 2987955