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MAB4349 Anti-TRA-2-49 Antibody, Liver/Bone/Kidney Alkaline Phosphatase, clone TRA-2-49/6E

MAB4349
100 µg  
Purchase on Sigma-Aldrich

Speciální nabídky

Přehled

Speciální nabídky

Tabulka spec. kláve

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, Po, Rb, FeFC, IF, ICC, IPMPurifiedMonoclonal Antibody
Description
Catalogue NumberMAB4349
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-TRA-2-49 Antibody, Liver/Bone/Kidney Alkaline Phosphatase, clone TRA-2-49/6E
Background InformationIn humans, four isoforms of alkaline phosphatase exist: intestinal, placental, placenta-like (germ cell type) and tissue-nonspecific. The tissue-nonspecific isoform of alkaline phosphatase is present in bone, liver and kidney; it differs from the other forms by posttranslational modification.
Undifferentiated human Embryonal Carcinoma (EC) and Embryonic Stem (ES) cells have been shown to express very high levels of Alkaline Phosphatase isozyme that is indistinguishable from the isozyme found in liver, bone and kidney. Expression levels of AP decrease following stem cell differentiation. This clone can be used to monitor the expression of the Human Liver / Bone / Kidney isozyme of Alkaline Phosphatase (AP), and hence the differentiation status of human EC and ES cells by flow cytometry.
Product Information
FormatPurified
Control
  • Undifferentiated human embryonic stem cells (H9 line). NTERA-2 cl.D1 whole cell lysate (pluripotent stem cells derived from teratocarcinoma and are considered the malignant counterparts of human embryonic stem cells)
PresentationPurified mouse monoclonal IgG1 liquid in buffer containing 0.02 M PBS, 0.25 M NaCl pH 7.6 with 0.1% Sodium Azide
Quality LevelMQ100
Applications
ApplicationThis Anti-TRA-2-49 Antibody, Liver/Bone/Kidney Alkaline Phosphatase, clone TRA-2-49/6E is validated for use in FC, IF, IC, IP for the detection of TRA-2-49.
Key Applications
  • Flow Cytometry
  • Immunofluorescence
  • Immunocytochemistry
  • Immunoprecipitation
Application NotesImmunocytochemistry: A previous lot was used on IC.

Immunofluorescence: A previous lot of this antibody was used in IF.

FACS Analysis: A starting range of 5-20 µg/mL is suggested.

Immunoprecipitation: A previous lot of this antibody was used in IP.

Optimal working dilutions must be determined by end user.
Biological Information
Immunogen2102Ep human Embryonal Carcinoma Cells
EpitopeLiver/Bone/Kidney Alkaline Phosphatase
Cloneclone TRA-2-49/6E
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityUndifferentiated human Embryonal Carcinoma (EC) and Embryonic Stem (ES) cells have been shown to express very high levels of Alkaline Phosphatase isozyme that is indistinguishable from the isozyme found in liver, bone and kidney. Expression levels of AP decrease following stem cell differentiation. This clone can be used in flow cytometry to determine the expression of the Liver / Bone / Kidney isozyme of Alkaline Phosphatase (AP), and hence monitor the differentiation status of EC and ES cells.
IsotypeIgG1
Species Reactivity
  • Human
  • Pig
  • Rabbit
  • Feline
Species Reactivity NoteReactivity has been demonstrated with Liver/ Bone/Kidney isozyme of Alkaline Phosphatase (AP) from Humans, Higher Primates (Chimpanzee, Gorilla, Orangutan and Gibbon), New World Monkeys (Owl Monkey, Squirrel Monkey), Cat, Pig, Rabbit and Tiger. No reactivity has been observed with Old World Monkeys.
This antibody does not cross react with other isozymes of AP.
Antibody TypeMonoclonal Antibody
Gene Symbol
  • TRA-2-49
Purification MethodProtein A Purfied
Stem Cell Type
  • Human Embryonic Stem Cells
  • Induced Pluripotent Stem Cells
Molecular Weight212/80/55 kDa.
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at from date of receipt.
Packaging Information
Material Size100 µg
Global Trade Item Number
Katalogové číslo GTIN
MAB4349 04053252267482