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IM82 Anti-TIMP-2 (Ab-4) Mouse mAb (T2-N IC3)

Anti-TIMP-2 (Ab-4) Mouse mAb (T2-N IC3) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Tissue Inhibitor of Metalloproteinase-2

IM82
  
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Přehled

Replacement Information

Tabulka spec. kláve

Host
M
Description
Overview

This product has been discontinued.





Recognizes the ~21-28 kDa TIMP-2 protein conditioned medium from dexamethasone-treated HT1080 cells, melanoma, A2058 cells, and bladder, breast, and ovarian carcinoma.
Catalogue NumberIM82
Brand Family Calbiochem®
SynonymsAnti-Tissue Inhibitor of Metalloproteinase-2
References
ReferencesCottam, D.W. and Rees, R.C. 1993. Intl. J. Oncol. 2, 861.
Stetler-Stevenson, W.G., et al. 1993. FASEB J. 7, 1434.
Woessner, J.F. 1991. FASEB J. 5, 2145.
Liotta, L.A. and Stetler-Stevenson, W.G. 1990. In Seminars in Cancer Biology, ed. M.M. Gottesman. Vol. 1, 99.
Product Information
DeclarationNot available for sale in Japan.
FormLiquid
FormulationIn 10 mM PBS, 0.2% BSA, pH 7.4.
Positive controlConditioned medium from dexamethasone-treated HT-1080 cells, melanoma A2058 cells, or bladder, breast, or ovarian carcinomas
Preservative≤0.1% sodium azide
Applications
Key Applications Affinity Purification
Frozen Sections
Immunoblotting (Western Blotting)
Paraffin Sections
Application NotesAffinity Purification (see comments)
Frozen Sections (1:50-1:100)
Immunoblotting (1:200-1:400, chemiluminescence)
Paraffin Sections (1:50-1:100, heat/pressure cooker pre-treatment required)
Application CommentsThis antibody has been reported to work for affinity purification methods. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogennative, human TIMP-2
ImmunogenHuman
CloneT2-N IC3
HostMouse
IsotypeIgG₁
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Ok to freeze
Special InstructionsFor long-term storage, aliquot and freeze (-20°C). Avoid freeze/thaw cycles.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
IM82 0

Documentation

References

Přehled odkazů
Cottam, D.W. and Rees, R.C. 1993. Intl. J. Oncol. 2, 861.
Stetler-Stevenson, W.G., et al. 1993. FASEB J. 7, 1434.
Woessner, J.F. 1991. FASEB J. 5, 2145.
Liotta, L.A. and Stetler-Stevenson, W.G. 1990. In Seminars in Cancer Biology, ed. M.M. Gottesman. Vol. 1, 99.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision04-September-2007 RFH
SynonymsAnti-Tissue Inhibitor of Metalloproteinase-2
ApplicationAffinity Purification (see comments)
Frozen Sections (1:50-1:100)
Immunoblotting (1:200-1:400, chemiluminescence)
Paraffin Sections (1:50-1:100, heat/pressure cooker pre-treatment required)
DescriptionPurified mouse monoclonal antibody derived by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with p3-X63-Ag8.653 mouse myeloma cells. Recognizes the ~21-28 kDa TIMP-2 protein.
BackgroundMatrix metalloproteinases (MMPs) are a family of enzymes that are responsible for the degradation of extracellular matrix components such as collagen, laminin and proteoglycans. In addition to sequence homology, all MMPs share the following characteristics: the catalytic mechanism is dependent upon a zinc ion at the active center, they cleave one or more extracellular matrix components, they are secreted as zymogens which are activated by removal of an ~10 kDa segment from the N-terminus and they are inhibited by tissue inhibitor of metalloproteinases (TIMP). These enzymes are involved in normal physiological processes such as embryogenesis and tissue remodeling and may play an important role in angiogenesis, arthritis, periodontitis, and metastasis. A family of endogenous inhibitors, tissue inhibitors of metalloproteinase (TIMP), regulate the activation and activity of MMPs. TIMPs are capable of altering the metastatic potential of cancer cells and have been shown to inhibit invasion and metastasis in animal models. TIMP-2 is a 194 amino acid unglycosylated protein of 21 kDa with 43% and 44% sequence homology to TIMP-1 and TIMP-3, respectively. TIMP-2 inhibits the activity of all active MMPs and regulates the activation of pro-MMP-2 by binding to the C-terminal region of pro-MMP-2 (Kd ~5 nM). As with TIMP-1, TIMP-2 has been shown to have erythroid potentiating activity and cell growth-promoting activity.
HostMouse
Immunogen speciesHuman
Immunogennative, human TIMP-2
CloneT2-N IC3
IsotypeIgG₁
Specieshuman
Positive controlConditioned medium from dexamethasone-treated HT-1080 cells, melanoma A2058 cells, or bladder, breast, or ovarian carcinomas
FormLiquid
FormulationIn 10 mM PBS, 0.2% BSA, pH 7.4.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsThis antibody has been reported to work for affinity purification methods. Antibody should be titrated for optimal results in individual systems.
Storage +2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsFor long-term storage, aliquot and freeze (-20°C). Avoid freeze/thaw cycles.
Toxicity Standard Handling
ReferencesCottam, D.W. and Rees, R.C. 1993. Intl. J. Oncol. 2, 861.
Stetler-Stevenson, W.G., et al. 1993. FASEB J. 7, 1434.
Woessner, J.F. 1991. FASEB J. 5, 2145.
Liotta, L.A. and Stetler-Stevenson, W.G. 1990. In Seminars in Cancer Biology, ed. M.M. Gottesman. Vol. 1, 99.