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MABE1923-100UG Anti-T2A Antibody, clone 5E6

Anti-T2A, clone 5E6, Cat. No. MABE1923, is a rat monoclonal antibody that detects T2A and is tested for use in ELISA, Immunofluorescence, Immunohistochemistry, and Western Blotting.

MABE1923-100UG
100 μg  
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Description
Catalogue NumberMABE1923-100UG
DescriptionAnti-T2A Antibody, clone 5E6
Alternate Names
  • Thosea asigna virus 2A
Background InformationThosea asigna virus (TaV), a member of the genus Betatetravirus of the family Tetraviridae, is a positive-sense single-stranded RNA virus that infects lepidopteran insects. This family is divided into the Betatetravirus and Omegatetravirus genera, based upon of the appearance of the virion and genome organization. 2A peptides and 2A-like peptide sequences (known as cis-acting hydrolase elements or CHYSEL) are a superior alternative to internal ribosomal entry sites (IRES) for coordinating the expression of multiple gene products from a single recombinant construct. The 2A sequences are relatively short peptides of about 16-20 amino acids (depending on the virus of origin). They contain a highly conserved sequence, GDVEXNPGP, at the C-terminus, which is shared by different 2As and is essential for the creation of steric hindrance and ribosome skipping. These peptides allow multiple proteins to be encoded as polyproteins, which then dissociate into component proteins upon translation. The 2A sequence impairs normal peptide bond formation via a mechanism called ribosomal skipping, which results in effective, non-enzymatic generation of distinct peptide products from a single multi-cistronic construct. These peptides are used by several families of viruses, the best-known amongst these are foot-and-mouth disease virus 2A (F2A) and Thosea asigna virus 2A (T2A). Clone 5E6 is a rat monoclonal antibody that reacts with a protein in lysate from HEK293T cells transfected with various 2A-tagged proteins.
References
Product Information
FormatPurified
PresentationPurified rat monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-T2A, clone 5E6, Cat. No. MABE1923, is a rat monoclonal antibody that detects T2A and is tested for use in ELISA, Immunofluorescence, Immunohistochemistry, and Western Blotting.
Key Applications
  • Western Blotting
  • Immunofluorescence
  • Immunohistochemistry
  • ELISA
Application NotesTested Applications

Western Blotting Analysis: A representative lot detected T2A tagged proteins in Western Blotting applications (Lange, A., et al. (2012). Differentiation.;83(2):S105-13).

Immunofluorescence Analysis: A 1:200 dilution of this antibody detected T2A tagged proteins in mouse E14.5 lung and pancreas tissue sections (Courtesy of Dr. Ingo Burtscher, Ph.D., Helmholtz Zentrum München, Germany).

Immunohistochemistry Applications: A representative lot detected T2A tagged proteins in Immunohistochemistry applications (Lange, A., et al. (2012). Differentiation.;83(2):S105-13).

Immunofluorescence Analysis: A representative lot detected T2A tagged proteins in Immunofluorescence applications (Lange, A., et al. (2012). Differentiation.;83(2):S105-13).

ELISA Analysis: A representative lot detected T2A tagged protein in ELISA applications (Lange, A., et al. (2012). Differentiation.;83(2):S105-13).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenOvalbumin-conjugated linear peptide corresponding to 17 amino acids from Thosea asigna T2A protein.
EpitopeC-terminal
Clone5E6
Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostRat
SpecificityClone 5E6 is a rat monoclonal antibody that detects T2A tagged protein.
IsotypeIgG1κ
Species Reactivity
  • All
Species Reactivity NoteAll.
Antibody TypeMonoclonal Antibody
Purification MethodProtein G purified
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in lysate from HEK293 cells transfected with alpha-Tubulin vector.

Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected T2A tagged alpha-Tubulin in lysate from HEK293 cells transfected with alpha-Tubulin vector.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
MABE1923-100UG 04065266908213

Documentation

Anti-T2A Antibody, clone 5E6 MSDS

Title

Safety Data Sheet (SDS) 

Anti-T2A Antibody, clone 5E6 Certificates of Analysis

TitleLot Number
Anti-T2A, clone 5E6 - 3902976 3902976
Anti-T2A, clone 5E6 - 3983454 3983454
Anti-T2A, clone 5E6 - 4004386 4004386
Anti-T2A, clone 5E6 - 4015058 4015058
Anti-T2A, clone 5E6 - 4023770 4023770
Anti-T2A, clone 5E6 - 4030998 4030998
Anti-T2A, clone 5E6 - 4060704 4060704
Anti-T2A, clone 5E6 - 4062762 4062762
Anti-T2A, clone 5E6 - 4073665 4073665
Anti-T2A, clone 5E6 - 4083072 4083072
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