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ABE284 Anti-T-box transcription factor TBX5 Antibody

ABE284
100 µL  
Purchase on Sigma-Aldrich

Speciální nabídky

Přehled

Speciální nabídky

Tabulka spec. kláve

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, RWB, ICC, IHCRbSerumPolyclonal Antibody
Description
Catalogue NumberABE284
DescriptionAnti-T-box transcription factor TBX5 Antibody
Alternate Names
  • T-box transcription factor TBX5
  • T-box protein 5
Background InformationThe gene encoding T-box transcription factor 5 (TBX5) is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene is closely linked to related family member T-box 3 (ulnar mammary syndrome) on human chromosome 12. The encoded protein may play a role in heart development and specification of limb identity.
Product Information
FormatSerum
Control
  • HeLa cell lysate
PresentationRabbit polyclonal serum with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-T-box transcription factor TBX5 Antibody detects level of T-box transcription factor TBX5 & has been published & validated for use in Western Blotting, ICC & IHC.
Key Applications
  • Western Blotting
  • Immunocytochemistry
  • Immunohistochemistry
Application NotesImmunocytochemistry Analysis: A representative lot from an independent laboratory detected T-box transcription factor TBX5 in 293T cells overexpressing T-box transcription factor TBX5, mouse primary cardiocytes treated with serum, HA-tagged Tbx5 transfected NIH/3T3 cells, and HA-tagged Tbx5 transfected C2C12 cells (Georges, R., et al. (2008). Mol Cell Biol. 28(12):4052-4067.)

Immunohistochemistry Analysis: A representative lot from an independent laboratory detected T-box transcription factor TBX5 in mouse embryonic tissue and in Tbx5 transgenic mouse heart tissues treated with tamoxifin (Georges, R., et al. (2008). Mol Cell Biol. 28(12):4052-4067.).
Biological Information
ImmunogenRecombinant protein corresponding to mouse T-box transcription factor TBX5.
HostRabbit
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • Tbx5
Purification MethodUnpurified
UniProt Number
Molecular Weight~53 kDa observed. An uncharacterized band may be observed at ~75 kDa in some cell lysates. Three isoforms due to alternative splicing may be observed at ~35 kDa, ~50 kDa, and ~64 kDa in some cell lysates. (Georges, R., et al. (2008). Mol Cell Biol. 28(12):4052-4067.).
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: A 1:1,000 dilution from a representative lot detected T-box transcription factor TBX5 in 10 µg of HeLa cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Global Trade Item Number
Katalogové číslo GTIN
ABE284 04053252819018