Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

ABS574-AF488 Anti-Surfactant Protein D Antibody, Alexa Fluor® 488 Conjugate

Detect Surfactant Protein D using this rabbit polyclonal Anti-Surfactant Protein D Antibody, Alexa Fluor® 488 Conjugate, Cat. No. ABS574-AF488, validated for use in Immunocytochemistry and Immunofluorescence.

Anti-Surfactant Protein D Antibody, Alexa Fluor® 488 Conjugate MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Gene Symbol: SFTPD, COLEC7, PSPD, SFTP4 UniProt Number: P35247
ABS574-AF488
100 µL  
Purchase on Sigma-Aldrich

Speciální nabídky

Přehled

Replacement Information

Speciální nabídky

100% Performance Guaranteed

Tabulka spec. kláve

Species ReactivityKey ApplicationsHostFormatAntibody Type
HICC, IFRbAlexaFluor®488Polyclonal Antibody
Description
Catalogue NumberABS574-AF488
DescriptionAnti-Surfactant Protein D Antibody, Alexa Fluor® 488 Conjugate
Alternate Names
  • Pulmonary surfactant-associated protein D
  • Collectin-7
  • Lung surfactant protein D
  • PSP-D
  • SP-D
Background InformationPulmonary surfactant-associated protein D (UniProt P35247; also known as Collectin-7, Lung surfactant protein D, PSP-D, SP-D) is encoded by the SFTPD (also known as COLEC7, PSPD, SFTP4) gene (Gene ID 6441) in human. Surfactant, produced by alveolar type II cells and airway Clara cells, resides at the air-liquid interface of the lungs and is most commonly known for its role in reducing surface tension. Surfactant is a lipoprotein complex composed of approximately 10% proteins, including surfactant protein A (SP-A), SP-B, SP-C, and SP-D. SP-A and SP-D belong to the collectin family of proteins and can modulate innate immunity. SP-D is known to enhance pulmonary clearance of pathogens, such as Pseudomonous aerginosa, Klebsiella pneumonia, respiratory syncytial virus (RSV) and Influenza virus. SP-D is also shown to modify allergic responsee in the lungs by binding common allergens, including house dust mite and pollen particles. SP-A-deficient and SP-D-deficient mice are susceptible to bleomycin-induced lung injury and display increased cellular inflammation, more severe lung fibrosis, and reduced survival. In addition to lung, SP-D is also reported to be detected in extrapulmonary tissues, such as kidney and pancreas. Serum levels of either SP-A or SP-D in human are correlated with mortality in patients with pulmonary fibrosis. Human SP-D is produced with a signal peptide sequence (a.a. 1-20), which is removed posttranslationally to yield the mature protein (a.a. 21-375) that contains a collagen-like domain (a.a. 46-222), a coiled coil region (a.a. 223-252), and a C-type lectin domain (a.a. 260-375).
References
Product Information
FormatAlexaFluor®488
PresentationPurified rabbit polyclonal antibody conjugate in PBS with 1.5% BSA and 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect Surfactant Protein D using this rabbit polyclonal Anti-Surfactant Protein D Antibody, Alexa Fluor® 488 Conjugate, Cat. No. ABS574-AF488, validated for use in Immunocytochemistry and Immunofluorescence.
Key Applications
  • Immunocytochemistry
  • Immunofluorescence
Application NotesImmunofluorescence Analysis: A 1:50 dilution from a representative lot detected surfactant protein D in human lung tissue sections.

The unconjugated antibody (Cat. No. ABS574) is also available for immunocytochemistry and Western blotting applications.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to a sequence within the C-type lectin domain of human surfactant protein D.
Epitopedomain of:
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis Alexa Fluor® 488-conjugated polyclonal antibody targets a sequence within the C-type lectin domain of human surfactant protein D.
Species Reactivity
  • Human
Species Reactivity NoteHuman. Target sequence shares 85.7% amino acid identity with the corresponding murine and rat region.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • SFTPD
  • COLEC7
  • PSPD
  • SFTP4
Purification MethodAffinity purified.
UniProt Number
Molecular Weight~48 kDa observed. 35.50/37.73 kDa (mature/pro-form) calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunocytochemistry in A549 cells.

Immunohistochemistry Analysis: A 1:100 dilution of this antibody detected surfactant protein D in A549 cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
ABS574-AF488 04054839086144