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MABC1211 Anti-Sorting Nexin-17 Antibody, clone 4B8.1

This Anti-Sorting Nexin-17 Antibody, clone 4B8.1 is validated for use in Western Blotting, Immunohistochemistry for the detection of Sorting Nexin-17.

Anti-Sorting Nexin-17 Antibody, clone 4B8.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Research Sub-Category: Vesicular Trafficking Gene Symbol: SNX17, KIAA0064 UniProt Number: Q15036
MABC1211
100 μg  
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Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IHCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABC1211
DescriptionAnti-Sorting Nexin-17 Antibody, clone 4B8.1
Alternate Names
  • Sorting nexin-17
  • SNX17
Background InformationSorting nexin-17 (UniProt Q15036) is encoded by the SNX17 (also known as KIAA0064) gene (Gene ID 9784) in human. The sorting nexins (SNXs) constitute a family of adaptor proteins involved in proteins trafficking through the endosomal pathway. Originally identified as a binding partner of the cell adhesion protein P-selectin, sorting nexin-17 (SNX17) has since been shown to mediate internalization and recycling of multiple cell surface proteins including P-selectin, amyloid precursor protein (APP), integrin β1, low density lipoprotein receptor (LDLR), LDLR related protein (Lrp1), ApoER2, and FEEL1. All SNX proteins contain a conserved phox homology (PX) domain that mediates phosphoinositides binding in a SNX type-specific manner, as well as various scaffolding modules including SH3, BAR, and PDZ domains. SNX17 PX domain (a.a. 1-109) displays specificity for phosphatidylinositol 3-monophosphate, which is enriched at the cytoplasmic face of the early endosome. SNX17 also contains a 4.1 protein/ezrin/radixin/moesin (FERM) domain (a.a. 115-432) comprised of an ubiquitin/Ras association (RA) subdomain known as the F1 lobe (a.a. 113-205), an F2 lobe (a.a. 206-269) with a helical structure similar to acyl-CoA binding protein, and an F3 lobe (a.a. 270-388) similar to the phosphotyrosine binding (PTB)/pleckstrin homology domain. The FERM domain mediates interaction with Asn-Pro-Xaa-Tyr/Phe (NPX(Y/F) motif-containing proteins for internalization as well as interaction with adaptor proteins-containing PTB (or FERM) domains to trigger signaling events.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-Sorting Nexin-17 Antibody, clone 4B8.1 is validated for use in Western Blotting, Immunohistochemistry for the detection of Sorting Nexin-17.
Key Applications
  • Western Blotting
  • Immunohistochemistry
Application NotesImmunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Sorting Nexin-17 in human pancreas, prostate, and cerebral cortex tissue sections.
Biological Information
ImmunogenGST-tagged recombinant fragment from the FERM-like region of human Sorting Nexin-17.
EpitopeWithin the FERM-like region.
Clone4B8.1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 4B8.1 recognizes an internal epitope within the FERM-like region present in both spliced isoforms of human sorting nexin-17 reported by UniProt (Q15036).
IsotypeIgG2bκ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • SNX17
  • KIAA0064
Purification MethodProtein G purified
UniProt Number
Molecular Weight~52 kDa observed. 52.90 kDa (isoform 1) and 50.00 kDa (isoform 2) calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in U251 cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Sorting Nexin-17 in 10 µg of U251 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
MABC1211 04055977353532