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ABC1454-25UL Anti-Sodium-Iodide Symporter Antibody, C-terminal 2

Detect Sodium/iodide cotransporter using this rabbit polyclonal Anti-Sodium-Iodide Symporter, C-terminal 2, Cat. No. ABC1454-25UL, validated for use in Immunohistochemistry (Paraffin), Immunocytochemistry and Western Blotting,

Research Category: Apoptosis & Cancer Gene Symbol: SLC5A5, NIS UniProt Number: Q92911
ABC1454-25UL
25 µL  
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Tabulka spec. kláve

Species ReactivityKey ApplicationsHostFormatAntibody Type
HIH(P), ICC, WBRbPurifiedPolyclonal Antibody
Description
Catalogue NumberABC1454-25UL
DescriptionAnti-Sodium-Iodide Symporter Antibody, C-terminal 2
Alternate Names
  • Sodium/iodide cotransporter
  • Na(+)/I(-) cotransporter
  • Na(+)/I(-) symporter
Background InformationSodium/iodide cotransporter (UniProt: Q92911; also known as Na(+)/I(-) cotransporter, Sodium-iodide symporter, Na(+)/I(-) symporter) is encoded by the SLC5A5 (also known as NIS) gene (Gene ID: 6528) in human. Sodium/iodide cotransporter is a multi-pass membrane protein that mediates iodide uptake in thyroid gland. It is expressed primarily in thyroid tissue, but is present in lower levels in mammary gland and ovary. It expression is significantly reduced in tumors. The presence of symporter in mammary glands leads to secretion of iodine in milk that is required for thyroid function in neonatal animals. NIS gene and protein expression is stimulated by thyroid-stimulating hormone. Both human and rat NIS contain N-glycosylation sites, however, glycosylation is not required for its full activity. Abnormalities in the expression of NIS gene lead to thyroid disease and can result in congenital hypothyroidism. Autoantibodies to NIS adversely affect iodide transport.
References
Product Information
FormatPurified
PresentationPurified rabbit polyclonal antibody in buffer containing  76 mM Tris, 192 mM glycine, pH 7.4   with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect Sodium/iodide cotransporter using this rabbit polyclonal Anti-Sodium-Iodide Symporter, C-terminal 2, Cat. No. ABC1454-25UL, validated for use in Immunohistochemistry (Paraffin), Immunocytochemistry and Western Blotting,
Key Applications
  • Immunohistochemistry (Paraffin)
  • Immunocytochemistry
  • Western Blotting
Application NotesImmunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected Sodium-Iodide Symporter in human thyroid and human salivary gland tissue sections.
Immunocytochemistry Analysis: A representative lot detected Sodium-Iodide Symporter in COS-7 cells transfected with wild-type human NIS and in COS-7 cells transfected with mutant Q267E human NIS (De la Vieja, A., et. al. (2005). Mol Endocrinol. 19(11):2847-58; De la Vieja, A., et. al. (2004). J Cell Sci. 117(Pt 5):677-87).
Immunohistochemistry Analysis: A representative lot detected Sodium-Iodide Symporter in Immunohistochemistry application (De la Vieja, A., et. al. (2004). J Cell Sci. 117(Pt 5):677-87; Wapnir, I.L., et. al. (2004). Clin Cancer Res. 10(13):4294-302; Wapnir, I.L., et. al. (2003). J Clin Endocrinol Metab. 88(4):1880-8).
Immunoprecipitation Analysis: A representative lot detected Sodium-Iodide Symporter in Biotinylated NIS constructs expressed in COS-7 cells (De la Vieja, A., et. al. (2004). J Cell Sci. 117(Pt 5):677-87).
Flow Cytometry Analysis: A representative lot detected Sodium-Iodide Symporter in COS-7 cells transfected with WT and mutant Q267E NIS (De la Vieja, A., et. al. (2004). J Cell Sci. 117(Pt 5):677-87).
Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected Sodium-Iodide Symporter in COS-7 cells transfected with hNIS ( Courtesy of Dr Nancy Carrasco & Dr. Andrea Reyna-Neyra  at Yale University ).
Western Blotting Analysis: A representative lot detected Sodium-Iodide Symporter in COS-7 cells transfected with NIS and COS-7 cells transfected with WT and mutant Q267E NIS (De la Vieja, A., et. al. (2005). Mol Endocrinol. 19(11):2847-58; De la Vieja, A., et. al. (2004). J Cell Sci. 117(Pt 5):677-87).
Immunohistochemistry Analysis: A representative lot detected Sodium-Iodide Symporter in IHC-P human salivary gland and breast tissues (Tazebay, U.H., et. al. (2000). Nat Med. 6(8):871-8).
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to 13 amino acids from the C-terminal region of human Sodium-iodide symporter.
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis rabbit polyclonal antibody detects Sodium-iodide symporter in human. It targets an epitope within 13 amino acids from the C-terminal end. For Western blotting application, please do not boil the sample to avoid any aggregation. When transfering protein to nitrocellulose membrane use of 0.05% (w/v) lithium dodecyl sufate is highly recommended in the transfer buffer.
IsotypeIgG
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypePolyclonal Antibody
Gene Symbol
  • SLC5A5
  • NIS
Purification MethodAffinity Purified
UniProt Number
Molecular Weight68.6 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunohistochemistry in human stomach tissue sections.

Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected Sodium-Iodide Symporter in human stomach tissue sections.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
ABC1454-25UL 04054839441615

Documentation