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PC347 Anti-Serotonin 3 (5-HT₃) Receptor (444-457) Rabbit pAb

This Anti-Serotonin 3 (5-HT₃) Receptor (444-457) Rabbit pAb is validated for use in Frozen Sections, Immunoblotting for the detection of Serotonin 3 (5-HT₃) Receptor (444-457).

Anti-Serotonin 3 (5-HT₃) Receptor (444-457) Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
PC347
  
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Přehled

Tabulka spec. kláve

Species ReactivityHostAntibody Type
M, RRbPolyclonal Antibody
Description
OverviewRecognizes the serotonin 3 (5-HT3) receptor in mouse and rat brain tissue.
Catalogue NumberPC347
Brand Family Calbiochem®
Application Data
Detection of human Serotonin 5-HT3 Receptor by staining frozen sections. Samples: Floating mouse brain fixed in 4% paraformaldehyde. Primary antibody: Anti-Serotonin (5-HT3) Receptor (444-457) Rabbit pAb (Cat. No. PC347) (5 µg/ml). Detection: DAB.
References
ReferencesMorales, M., et al. 1998. J. Comp. Neurol. 385, 385.
Morales, M., et al. 1996. Brain Res. 731, 199.
Morales, M., et al. 1996. Mol. Brain Res. 36, 251.
Teitler, M. and Herrick-Davis, K. 1994. Crit. Rev. Neurobiol. 8, 175.
Tecott, L.H., et al. 1993. Proc. Natl. Acad. Sci. USA 90, 1430.
Maricq, A.V., et al. 1991. Science 254, 432.
Pratt, G.D., et al. 1990. Trends Pharmacol. Sci. 11, 135.
Derkach, V., et al. 1989. Nature 33, 706.
Product Information
FormLiquid
Formulation25 µg antibody in 0.05 M sodium phosphate buffer, 0.2% gelatin
Positive controlMouse or rat brain tissue
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Frozen Sections
Not Western Blot
Application NotesFrozen Sections (2-5 µg/ml)
Immunoblotting (not recommended)
Application CommentsThis antibody is not recommended for immunoblotting using total mouse or rat brain extract. Immunohistochemistry was performed using floating mouse and rat brain sections fixed in 4% paraformaldehyde. Staining was completely abolished by preincubating the affinity purified antibody with control peptide at 10-6M. Unconjugated serotonin 5-HT3 receptor control peptide has been included for use as an internal control for antibody specificity. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena synthetic peptide (SLEKRDEMREVARD) corresponding to amino acids 444-457 of rat serotonin (5-HT₃) receptor, conjugated to KLH
ImmunogenRat
HostRabbit
IsotypeIgG
Species Reactivity
  • Mouse
  • Rat
Antibody TypePolyclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Global Trade Item Number
Katalogové číslo GTIN
PC347 0