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MABS2317-100UG Anti-SIRPA/CD172a Antibody, clone SE12C3

Anti-SIRPA/CD172a, clone SE12C3, Cat. No. MABS2317, is a mouse monoclonal antibody that detects SIRPA/CD172a and is tested for use in ELISA, Flow Cytometry, Immunocytochemistry, Inhibition, and Immunoprecipitation.

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Description
Catalogue NumberMABS2317-100UG
DescriptionAnti-SIRPA/CD172a Antibody, clone SE12C3
Alternate Names
  • Tyrosine-protein phosphatase non-receptor type substrate 1
  • SHP substrate 1
  • SHPS-1
  • Brain Ig-like molecule with tyrosine-based activation motifs
  • CD172 antigen-like family member A
  • Inhibitory receptor SHPS-1
  • Macrophage fusion receptor MyD-1 antigen
  • Signal-regulatory protein alpha-1
  • Sirp-alpha-1
  • Signal-regulatory protein alpha-2
  • Sirp-alpha-2
  • Signal-regulatory protein alpha-3
  • Sirp-alpha-3
  • p84
  • CD172a
Background InformationTyrosine-protein phosphatase non-receptor type substrate 1 (UniProt: P78324; also known as SHP substrate 1, SHPS-1, Brain Ig-like molecule with tyrosine-based activation motifs, Bit, CD172 antigen-like family member A, Inhibitory receptor SHPS-1, Macrophage fusion receptor, MyD-1 antigen, Signal-regulatory protein alpha-1, Sirp-alpha-1, Signal-regulatory protein alpha-2, Sirp-alpha-2, Signal-regulatory protein alpha-3, Sirp-alpha-3, p84, CD172a) is encoded by the SIRPA (also known as BIT, MFR, MYD1, PTPNS1, SHPS1, SIRP) gene (Gene ID: 140885) in human. SIRPA/CD172a is a single-pass type I membrane protein of the Ig superfamily that is ubiquitously expressed with high expression reported in brain. It is also detected on myeloid cells, but not on T cells. SIRPA is synthesized with a signal peptide (aa 1-30), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 31-373), a transmembrane domain (aa 374-394) and a cytoplasmic domain (aa 395-504). The extracellular region contains three Ig-like loops. The most distal loop (D1) contains and Ig-like V type domain (aa 32-137) whereas the two membrane proximal loops contain Ig-like C1 type domains (aa 148-247 and 254-348). SIRPA serves as an immunoglobulin-like cell surface receptor for CD47, and their interactions is reported to mediate negative regulation of several monocyte/ macrophage functions. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. SIRP alpha also acts as a docking protein and induces translocation of PTPN6, PTPN11, and other binding partners from the cytosol to the plasma membrane. SIRPA is phosphorylated on tyrosine residues in response to stimulation with EGF, growth hormone, insulin, and platelet derived growth factor. (Ref.: Murata, Y., et al., ( 2018). Cancer Sci. 109(5); 1300-1308; Lee, WY et al., (2007). J. Immunol.; 179(11); 7741-7750; Seiffert, M., et al (1999). Blood. 94(11); 3633-3643).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in PBS without preservatives.
Quality LevelMQ200
Applications
ApplicationAnti-SIRPA/CD172a, clone SE12C3, Cat. No. MABS2317, is a mouse monoclonal antibody that detects SIRPA/CD172a and is tested for use in ELISA, Flow Cytometry, Immunocytochemistry, Inhibition, and Immunoprecipitation.
Key Applications
  • Flow Cytometry
  • Inhibition
  • Immunoprecipitation
  • ELISA
  • Immunocytochemistry
Application NotesTested Applications

Immunoprecipitation Analysis: A representative lot immunoprecipitated SIRPA/CD172a in Immunoprecipitation applications (Seiffert, M., et al. (1999). Blood. 94(11):3633-43).

Inhibition Assay: A representative lot inhibited the binding of soluble SIRPA to cells. (Seiffert, M., et al. (1999). Blood. 94(11):3633-43; Murata, Y., et al. (2018). Cancer Sci. 109(5):1300-1308).

Immunocytochemistry Analysis: A representative lot detected SIRPA/CD172a in Immunocytochemistry applications (Seiffert, M., et al. (1999). Blood. 94(11):3633-43; Murata, Y., et al. (2018). Cancer Sci. 109(5):1300-1308).

ELISA Analysis: A representative lot detected SIRPA/CD172a in ELISA applications (Murata, Y., et al. (2018). Cancer Sci. 109(5):1300-1308).

Flow Cytometry Analysis: A representative lot detected SIRPA/CD172a in Flow Cytometry applications (Seiffert, M., et al. (1999). Blood. 94(11):3633-43).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biological Information
ImmunogenGST-tagged recombinant extracellular domain of human SIRPA/CD172a.
EpitopeExtracellular domain
CloneSE12C3
Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone SE12C3 is a mouse monoclonal antibody that detects SIRPA/CD172a. It targets an epitope within the extracellular domain.
IsotypeIgG1
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • SIRPA
Purification MethodProtein G purified
UniProt Number
Molecular Weight54.97 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Flow Cytometry in Human peripheral blood mononuclear cells (PBMC).

Flow Cytometry Analysis: 1.0 µg of this antibody detected SIRPA/CD172a in one million Human peripheral blood mononuclear cells (PBMC).
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
MABS2317-100UG 04065270937889