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ABN327 Anti-SCRAPPER Antibody

This SCRAPPER antibody is validated for use in WB & IHC for the detection of the SCRAPPER protein.

Anti-SCRAPPER Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Developmental Neuroscience Gene Symbol: FBXL20, SCRAPPER UniProt Number: Q96IG2
ABN327
100 µg  
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Speciální nabídky

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Tabulka spec. kláve

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IHCRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABN327
DescriptionAnti-SCRAPPER Antibody
Alternate Names
  • F-box
  • Leucine rich repeat protein 20
  • FBXL20
  • FBl2
  • FBL20
Background InformationMembers of the F-box protein family, such as SCRAPPER, are characterized by an approximately 40-amino acid F-box motif. SCRAPPER is selectively expressed in the brain, broadly expressed within the mouse CNS, and is abundant at the presynaptic membrane. It has orthologs in C. elegans, D. melanogaster, and mammals which suggests that it might function as an important membrane-localized E3 ligase in various species. SCRAPPER is a major presynaptic E3 ubiquitin ligase that acts through RIM1a via degradation and the ubiquitin-proteasome-system (UPS) pathway to critically regulate synaptic transmission. This identifies protein degradation as a mechanism for holding synaptic communication in check.
References
Product Information
FormatAffinity Purified
Control
  • Human spleen tissue lysate
PresentationPurified rabbit polyclonal in buffer containing PBS with 0.02% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis SCRAPPER antibody is validated for use in WB & IHC for the detection of the SCRAPPER protein.
Key Applications
  • Western Blotting
  • Immunohistochemistry
Application NotesWestern Blot Analysis: 1 µg/mL from a representative lot detected SCRAPPER in 10 µg/mL of RAW264.7 cell lysate.

Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected SCRAPPER in human midbrain tissue.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to human SCRAPPER.
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
Species Reactivity
  • Human
Antibody TypePolyclonal Antibody
Gene Symbol
  • FBXL20
  • SCRAPPER
Purification MethodAntigen Affinity Purified
UniProt Number
Molecular Weight~48 kDa observed. Uncharacterized bands may appear at ~52 kDa, ~58 kDa and ~70 kDa in some lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in human spleen tissue lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected SCRAPPER in 10 µg/mL of human spleen tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
ABN327 04053252831096