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MABE341-AF488 Anti-Puromycin, clone 17H1, Alexa Fluor® 488 Conjugate Antibody

This Anti-Puromycin, clone 17H1 is validated for use in Immunocytochemistry for the detection of Puromycin.

Anti-Puromycin, clone 17H1, Alexa Fluor® 488 Conjugate Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Cell Cycle, DNA Replication & Repair
MABE341-AF488
100 µL  
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Tabulka spec. kláve

Species ReactivityKey ApplicationsHostFormatAntibody Type
HICCRAlexaFluor®488Monoclonal Antibody
Description
Catalogue NumberMABE341-AF488
DescriptionAnti-Puromycin, clone 17H1, Alexa Fluor® 488 Conjugate Antibody
Background InformationPuromycin is an aminonucleoside antibiotic, derived from the Streptomyces alboniger bacterium, that functions as a protein synthesis inhibitor that blocks translation through premature chain termination in the ribosome. Monoclonal antibodies to puromycin may be used with standard immunochemical methods to directly monitor translation, a method known as surface sensing of translation (SUnSET). Part of the molecule resembles the 3' end of the aminoacylated tRNA, making it useful for protein translation analysis. Puromycin induces DNA fragmentation in thymocytes and in human HL-60 leukemia cells.
This product is a conjugated version of Cat. No. MABE341.
Product Information
FormatAlexaFluor®488
PresentationPurified rat monoclonal conjugate in buffer containing PBS and 15 mg/ml BSA with 0.1% Sodium Azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-Puromycin, clone 17H1 is validated for use in Immunocytochemistry for the detection of Puromycin.
Key Applications
  • Immunocytochemistry
Biological Information
ImmunogenPuromycin-incorporated neosynthesized proteins at multiple molecular weights
Clone17H1
Concentration0.5 mg/mL
HostRat
SpecificityThis antibody detects Puromycin-incorporated neosynthesized proteins.
Species Reactivity
  • Human
Species Reactivity NoteDemonstrated to react with Human test sample, preincubated with Puromycin. Predicted to react with all species when test sample is incubated with Puromycin.
Antibody TypeMonoclonal Antibody
Purification MethodProtein G Purified
Molecular WeightRefer to Cat. No. MABE341 for observed molecular weight information.
Product Usage Statements
Quality AssuranceEvaluated by Immunocytochemistry in untreated and Puromycin treated HeLa cells.

Immunocytochemistry Analysis: A 1:500 dilution of this antibody detected Puromycin-incorporated neosynthesized proteins in HeLa cells treated with Puromycin only.

Alexa Fluor® is a registered trademark of Life Technologies®.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Global Trade Item Number
Katalogové číslo GTIN
MABE341-AF488 04053252973895