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NA63 Anti-Polypyrimidine Tract Binding Protein (Ab-1) Mouse mAb (SH54)

This Anti-Polypyrimidine Tract Binding Protein (Ab-1) Mouse mAb (SH54) is validated for use in Immunoblotting, IF, Paraffin Sections for the detection of Polypyrimidine Tract Binding Protein (Ab-1).

Anti-Polypyrimidine Tract Binding Protein (Ab-1) Mouse mAb (SH54) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-PTB

NA63
  
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Přehled

Replacement Information

Tabulka spec. kláve

Species ReactivityHostAntibody Type
HMMonoclonal Antibody
Description
Overview

This product has been discontinued.



Recognizes ~57 kDa PTB protein in Jurkat cells.
Catalogue NumberNA63
Brand Family Calbiochem®
SynonymsAnti-PTB
Application Data
Detection of human polypyrimidine tract binding protein by immunoblotting. Sample: Whole cell lysate from Jurkat cells. Primary antibody: Anti-Polypyrimidine Tract Binding Protein (Ab-1) Mouse mAb (SH54) (Cat. No. NA63) (1 μg/ml). Detection: chemiluminescence.
References
References<→EMD:if(output-is PDS)→> Gontarek, R. R., et al. 1999. Nucleic Acids Res. 27, 1457.
Ito, T. and Lai, M. M. 1999. Virology 254, 288.
Wagner, E. J., et al. 1999. Electrophoresis 20, 1082.
Zhang, L, et al. 1999. RNA 5, 117.
Huang, S., et al. 1997. J. Cell Biol. 137, 965.
Valcarcel, J., and Gebauer, F. 1997. Curr. Biol. 7, R705.
Brunel, F., et al. 1996. Nucleic Acids Res. 24, 1608. <→EMD:endif()→>
Product Information
FormLiquid
FormulationIn 50 mM sodium phosphate, 0.2% gelatin.
Positive controlJurkat cells or breast carcinoma tissue
Preservative≤0.1% sodium azide
Applications
Application ReferencesImmunohistochemistry, Immunofluorescence, Original Clone Huang, S., et al. 1997. J. Cell Biol. 137, 965.
Key Applications Immunoblotting (Western Blotting)
Immunofluorescence
Paraffin Sections
Application NotesImmunoblotting (1 µg/ml)
Immunofluorescence (1-2 µg/ml, see application references)
Paraffin Sections (2.5 µg/ml, heat pre-treatment required see application references)
Application CommentsAntibody should be titrated for optimal results in individual systems.
Biological Information
ImmunogenHeLa cell nuclear extract
ImmunogenHuman
CloneSH54
HostMouse
IsotypeIgG₁
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
NA63 0

Documentation

Anti-Polypyrimidine Tract Binding Protein (Ab-1) Mouse mAb (SH54) MSDS

Title

Safety Data Sheet (SDS) 

Anti-Polypyrimidine Tract Binding Protein (Ab-1) Mouse mAb (SH54) Certificates of Analysis

TitleLot Number
NA63

References

Přehled odkazů
<→EMD:if(output-is PDS)→> Gontarek, R. R., et al. 1999. Nucleic Acids Res. 27, 1457.
Ito, T. and Lai, M. M. 1999. Virology 254, 288.
Wagner, E. J., et al. 1999. Electrophoresis 20, 1082.
Zhang, L, et al. 1999. RNA 5, 117.
Huang, S., et al. 1997. J. Cell Biol. 137, 965.
Valcarcel, J., and Gebauer, F. 1997. Curr. Biol. 7, R705.
Brunel, F., et al. 1996. Nucleic Acids Res. 24, 1608. <→EMD:endif()→>
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision05-September-2007 RFH
SynonymsAnti-PTB
ApplicationImmunoblotting (1 µg/ml)
Immunofluorescence (1-2 µg/ml, see application references)
Paraffin Sections (2.5 µg/ml, heat pre-treatment required see application references)
Application Data
Detection of human polypyrimidine tract binding protein by immunoblotting. Sample: Whole cell lysate from Jurkat cells. Primary antibody: Anti-Polypyrimidine Tract Binding Protein (Ab-1) Mouse mAb (SH54) (Cat. No. NA63) (1 μg/ml). Detection: chemiluminescence.
DescriptionPurified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with SP2/0 mouse myeloma cells (see application references). Recognizes the ~57 kDa PTB protein.
BackgroundPolypyrimidine tract binding protein (PTB) is an abundant vertebrate hnRNP protein that functions as a splicing regulator with multiple targets. PTB exists as a 57 kDa protein that is found exclusively in the perinucleolar compartment (PNC) and regulates alternative splicing by binding to a specific pyrimidine tract sequence that is typically found near the 3' end of introns. The PNC in turn is a structure that is more prevalent in cancer cells than in normal cells. This latter observation suggests the possibility of using expression of PTB as a marker for tumor cells. In solution phase, PTB exists as a homodimer and in some cancer cells exists as two isoforms, the ratio of which appears to correlate with metastatic potential particularly in prostatic carcinoma. PTB is involved in the regulation of many different viral genomes such as that of hepatitis C virus.
HostMouse
Immunogen speciesHuman
ImmunogenHeLa cell nuclear extract
CloneSH54
IsotypeIgG₁
Specieshuman, not mouse, not rat
Positive controlJurkat cells or breast carcinoma tissue
FormLiquid
FormulationIn 50 mM sodium phosphate, 0.2% gelatin.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsAntibody should be titrated for optimal results in individual systems.
Storage +2°C to +8°C
Do Not Freeze Yes
Toxicity Standard Handling
References<→EMD:if(output-is PDS)→> Gontarek, R. R., et al. 1999. Nucleic Acids Res. 27, 1457.
Ito, T. and Lai, M. M. 1999. Virology 254, 288.
Wagner, E. J., et al. 1999. Electrophoresis 20, 1082.
Zhang, L, et al. 1999. RNA 5, 117.
Huang, S., et al. 1997. J. Cell Biol. 137, 965.
Valcarcel, J., and Gebauer, F. 1997. Curr. Biol. 7, R705.
Brunel, F., et al. 1996. Nucleic Acids Res. 24, 1608. <→EMD:endif()→>
Application referencesImmunohistochemistry, Immunofluorescence, Original Clone Huang, S., et al. 1997. J. Cell Biol. 137, 965.