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PH01 Anti-PTP1B (Ab-1) Mouse mAb (FG6-1G)

This Anti-PTP1B (Ab-1) Mouse mAb (FG6-1G) is validated for use in Immunoblotting for the detection of PTP1B (Ab-1).

Anti-PTP1B (Ab-1) Mouse mAb (FG6-1G) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Protein Tyrosine Phosphatase 1B

PH01
  
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Přehled

Replacement Information

Tabulka spec. kláve

Host
M
Description
OverviewRecognizes the ~50 kDa PTPase 1B protein. Weak reactivity with mouse PTPase 1B and human TC/PTPase (a 48 kDa protein).

This product has been discontinued.
Catalogue NumberPH01
Brand Family Calbiochem®
SynonymsAnti-Protein Tyrosine Phosphatase 1B
References
ReferencesFlint, A.J., et al. 1993. EMBO J. 12, 1937.
Brown-Shimer, S., et al. 1992. Cancer Res. 52, 478.
Fischer, E.H., et al. 1991. Science 253, 401.
Pallen, C.J., et al. 1991. Biochem. J. 276, 315.
Brown-Shimer, S., et al. 1990. Proc. Natl. Acad. Sci. USA 87, 5148.
Chernoff, J., et al. 1990. Proc. Natl. Acad. Sci. USA 87, 2735.
Charbonneau, H., et al. 1989. Proc. Natl. Acad. Sci. USA 86, 5252.
Charbonneau, H., et al. 1988. Proc. Natl. Acad. Sci. USA 85, 7182.
Product Information
FormLiquid
FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
Positive controlBSC-40 cells
Preservative≤0.1% sodium azide
Applications
Key Applications Immunoblotting (Western Blotting)
Immunofluorescence
Immunoprecipitation
Application NotesImmunoblotting (1-5 µg/ml)
Immunofluorescence (1-5 µg/ml)
Immunoprecipitation (1-2 µg/sample)
Application CommentsThis antibody is weakly reactive to murine PTPase 1B and human TC PTPase (~48 kDa). PTPaseB1 undergoes reversible phosphorylation during mitosis; this is accompanied by a mobility shift on SDS-PAGE. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenrecombinant, human protein tyrosine phosphatase 1B
Immunogenhuman
Epitopewithin the catalytic domain
CloneFG6-1G
HostMouse
IsotypeIgG2a
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
PH01 0

Documentation

Anti-PTP1B (Ab-1) Mouse mAb (FG6-1G) Certificates of Analysis

TitleLot Number
PH01

References

Přehled odkazů
Flint, A.J., et al. 1993. EMBO J. 12, 1937.
Brown-Shimer, S., et al. 1992. Cancer Res. 52, 478.
Fischer, E.H., et al. 1991. Science 253, 401.
Pallen, C.J., et al. 1991. Biochem. J. 276, 315.
Brown-Shimer, S., et al. 1990. Proc. Natl. Acad. Sci. USA 87, 5148.
Chernoff, J., et al. 1990. Proc. Natl. Acad. Sci. USA 87, 2735.
Charbonneau, H., et al. 1989. Proc. Natl. Acad. Sci. USA 86, 5252.
Charbonneau, H., et al. 1988. Proc. Natl. Acad. Sci. USA 85, 7182.

Brochure

Title
Protein Phosphatases Technical Bulletin
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision16-October-2007 RFH
SynonymsAnti-Protein Tyrosine Phosphatase 1B
ApplicationImmunoblotting (1-5 µg/ml)
Immunofluorescence (1-5 µg/ml)
Immunoprecipitation (1-2 µg/sample)
DescriptionPurified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing mouse splenocytes with SP2/0 mouse myeloma cells. Recognizes the ~50 kDa PTPase 1B protein.
BackgroundProtein tyrosine phosphatases (PTPases) make up an expanding family of transmembrane and intracellular proteins, which function to remove phosphate groups from protein phosphoryl-tyrosine residues. PTPase 1B was initially identified as an active intracellular phosphatase from human placenta made up of 321 amino acids. It was subsequently shown to derive from a larger precursor protein containing 435 amino acids. Further examination failed to show alternative splicing or more than a single copy of the PTP1B in the genome. Purification of a 50,000 kDa protein with phosphatase activity from human placenta demonstrated the protein to have an amino acid composition similiar to that for PTPase 1B and N-terminal analysis revealed an identical sequence. PTPases are involved in regulating processes where protein kinases and protein tyrosine phosphorylation are important. Recently it has been demonstrated that expression of PTPase 1B has profound effects on the transformation induced by the human neu oncogene. Based on this and other observations it has been hypothesized that PTPases have the potential to function as tumor suppressor genes.
HostMouse
Immunogen specieshuman
Immunogenrecombinant, human protein tyrosine phosphatase 1B
Epitopewithin the catalytic domain
CloneFG6-1G
IsotypeIgG2a
Speciesavian, hamster, human, mouse (weakly), rat
Positive controlBSC-40 cells
FormLiquid
FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsThis antibody is weakly reactive to murine PTPase 1B and human TC PTPase (~48 kDa). PTPaseB1 undergoes reversible phosphorylation during mitosis; this is accompanied by a mobility shift on SDS-PAGE. Antibody should be titrated for optimal results in individual systems.
Storage +2°C to +8°C
Do Not Freeze Yes
Toxicity Standard Handling
ReferencesFlint, A.J., et al. 1993. EMBO J. 12, 1937.
Brown-Shimer, S., et al. 1992. Cancer Res. 52, 478.
Fischer, E.H., et al. 1991. Science 253, 401.
Pallen, C.J., et al. 1991. Biochem. J. 276, 315.
Brown-Shimer, S., et al. 1990. Proc. Natl. Acad. Sci. USA 87, 5148.
Chernoff, J., et al. 1990. Proc. Natl. Acad. Sci. USA 87, 2735.
Charbonneau, H., et al. 1989. Proc. Natl. Acad. Sci. USA 86, 5252.
Charbonneau, H., et al. 1988. Proc. Natl. Acad. Sci. USA 85, 7182.