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MABF3036-100UG Anti-PSGL-1/CD162 Antibody, clone HECA-452

Anti-PSGL-1/CD162, clone HECA-452, Cat. No. MABF3036, is a rat monoclonal antibody that detects P-selectin glycoprotein ligand 1 (PSGL-1) and is tested for use in Flow Cytometry, Immunohistochemistry, and Western Blotting.

MABF3036-100UG
100 μg  
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Replacement Information
Description
Catalogue NumberMABF3036-100UG
DescriptionAnti-PSGL-1/CD162 Antibody, clone HECA-452
Alternate Names
  • P-selectin glycoprotein ligand 1
  • PSGL-1 Selectin P ligand
  • ;CD162
  • P-selectin glycoprotein ligand 1
Background InformationP-selectin glycoprotein ligand 1 (UniProt: Q14242; also known as PSGL-1 Selectin P ligand, CD162) is encoded by the SELPLG gene (Gene ID: 6404) in human. Leukocytes express L-selectin ligands that are critical for leukocyte-leukocyte interactions at sites of inflammation. PSGL-1 is a predominant leukocyte L-selectin ligand that displays appropriate sialyl Lewis x (sLex)-like carbohydrate determinants for L-selectin recognition. It is a single-pass, mucin-like type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-17), which is subsequently cleaved off to produce a form that contains an extracellular domain (18-320), a transmembrane domain (321-341), and a cytoplasmic domain (342-412). The mature form is generated following the cleaved of the propeptide (aa 18-41) region. PSGL-1 is a SLe(x)-type proteoglycan, which through high affinity, calcium-dependent interactions with E-, P- and L-selectins mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation. It is constitutively expressed on all human peripheral-blood T cells and is also expressed on skin-homing T cells. Clone HECA-452 can detect a human endothelial cell differentiation antigen (HECA) selectively expressed on high endothelium. In addition to reacting with high endothelium, it also shows some cross-reactivity with a set of monocytic cells. This clone is shown to block the majority of P-selectin binding to neutrophils. (Ref.: Tu, L., et al. (1999). J. Immunol. 163(9); 5070-5078; Fuhlbrigge, RC., et al. (1997). Nature. 389(6654); 978-981; Duivestijn, AM., et al. (1988). Am. J. Pathol. 130(1); 147-155).
References
Product Information
FormatPurified
Control
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PresentationPurified rat monoclonal antibody IgM in PBS without azide.
Quality LevelMQ200
Applications
ApplicationAnti-PSGL-1/CD162, clone HECA-452, Cat. No. MABF3036, is a rat monoclonal antibody that detects P-selectin glycoprotein ligand 1 (PSGL-1) and is tested for use in Flow Cytometry, Immunohistochemistry, and Western Blotting.
Key Applications
  • Flow Cytometry
  • Western Blotting
  • Immunohistochemistry
Application NotesTested applications

Immunohistochemistry Applications: A representative lot detected PSGL-1/CD162 in Immunohistochemistry application (Duijvestijn, A.M., et al. (1988). Am J Pathol. 130(1):147-55).

Flow Cytometry Analysis: A representative lot detected PSGL-1/CD162 in Flow Cytometry application (Picker, L.J., et al. (1991). Nature. 349(6312):796-9; Fuhlbrigge, R.C., et al. (1997). Nature. 389(6654):978-81).

Western Blotting Analysis: A representative lot detected PSGL-1/CD162 in Western Blotting application (Fuhlbrigge, R.C., et al. (1997). Nature. 389(6654):978-81).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biological Information
ImmunogenStromal preparation from human tonsil tissue.
EpitopeExtracellular domain
CloneHECA-452
Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostRat
SpecificityClone HECA-452 is a rat monoclonal antibody that detects PSGL-1/CD162. It targets an epitope within the extracellular domain.
IsotypeIgMκ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • SELPLG
Purification MethodLigatrap
UniProt Number
Molecular Weight43.2 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Flow Cytometry in Human peripheral blood mononuclear cells (PBMC).

Flow Cytometry Analysis (FC): 1.0 µg of this antibody stained one million Human peripheral blood mononuclear cells (PBMC).
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Katalogové číslo GTIN
MABF3036-100UG 04065269049135