Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

OP128 Anti-PLZF Mouse mAb (2A9)

OP128
  
Purchase on Sigma-Aldrich

Přehled

Tabulka spec. kláve

Host
M
Description
OverviewRecognizes the ~81 kDa PLZF in myelodysplastic cells treated with A23187 (Cat. No. 100105).

This product has been discontinued.





Catalogue NumberOP128
Brand Family Calbiochem®
SynonymsAnti-Promyelocytic Leukemia Zinc Finger
References
ReferencesYeyati, P.L., et al. 1999. Oncogene 18, 925.
Shaknovich, R., et al. 1998 Mol. Cell Biol. 18, 5533.
Li, J.Y., et al. 1997. Biol. Chem. 272, 22447.
Licht, J.D., et al. 1996. Oncogene 12, 323.
Avantaggiato, V., et al. 1995. J. Neurosci. 15, 4927.
Cook, M., et al. 1995. Proc. Natl. Acad. Sci. USA 92, 2249.
Reid, A., et al. 1995. Blood 86, 4544.
Chen, Z., et al. 1993. EMBO. J. 12,1161.
Product Information
FormLiquid
FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
Negative controlHEK293 or CV-1 cells
Positive controlHL-60 or KG1 cells
Preservative≤0.1% sodium azide
Applications
Application ReferencesOriginal Clone Shaknovich, R., et al. 1998 Mol. Cell Biol. 18, 5533.
Key Applications Gel Shift
Immunoblotting (Western Blotting)
Immunofluorescence
Immunoprecipitation
Application NotesGel Shift (2 µg)
Immunoblotting (2 µg/ml)
Immunofluorescence (2 µg/ml)
Immunoprecipitation (2 µg)
Application CommentsPLZF, which migrates on SDS/PAGE as an 81 kDa protein, is expressed at low levels in HL60 cells but at higher levels in myelodysplastic cells treated with the calcium ionophore A23187. Immunoprecipitation of PLZF from total cell lysate using Cat. No. OP128 followed by immunoblotting with Cat. No. OP128 can be used to detect low level expression of PLZF. The PLZF protein can be detected in human erythroleukemia cells and in CD34+ progenitor cells in addition to HL60 and KG1 cells. Anti-PLZF will also detect the PLZF-RARα fusion protein expressed in APL cells carrying the t(11;17)(q23;q21) translocation but not the RARα-PLZF fusion protein generated by the reciprocal translocation.(J. Licht, per. comm.) Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena recombinant protein consisting of human PLZF fused to GST
ImmunogenHuman
Epitopewithin amino acids 100-200 of PLZF
Clone2A9
HostMouse
IsotypeIgG2a
Concentration Label Please refer to vial label for lot-specific concentration
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Global Trade Item Number
Katalogové číslo GTIN
OP128 0